细胞生物学

分类

    A Semi-quantitative Scoring System for Green Histopathological Evaluation of Large Animal Models of Acute Lung Injury
    用于急性肺损伤大型动物模型绿色组织病理学评价的半定量评分系统

    Acute respiratory distress syndrome (ARDS) is a life-threatening, high mortality pulmonary condition characterized by acute lung injury (ALI) resulting in diffuse alveolar damage. Despite progress regarding the understanding of ARDS pathophysiology,

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    Identification and Quantitation of Neutrophil Extracellular Traps in Human Tissue Sections
    人体组织切片中中性粒细胞胞外陷阱的鉴定与定量
    作者:  Coraline Radermecker, Alexandre Hego, Philippe DelvenneThomas Marichal, 日期: 09/20/2021, 浏览量: 2747, Q&A: 0

    Neutrophils are one of the first innate immune cells recruited to tissues during inflammation. An important function of neutrophils relies on their ability to release extracellular structures, known as Neutrophil Extracellular Traps or NETs, into

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    Histological and Immunohistochemical Examination of Stem Cell Proliferation and Reepithelialization in the Wounded Skin
    损伤皮肤干细胞增殖和再生的组织学和免疫组化检测
    作者:  Rupali Gund, Ravindra Zirmire, Haarshaadri J., Gaurav KansagaraColin Jamora, 日期: 01/20/2021, 浏览量: 4214, Q&A: 0

    The skin is the largest organ that protects our body from the external environment and it is constantly exposed to pathogenic insults and injury. Repair of damage to this organ is carried out by a complex process involving three overlapping phases

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    Staining and Quantitative Analysis of Myelinating Oligodendrocytes in the Mouse Grey Matter
    小鼠灰质髓鞘少突胶质细胞的染色及定量分析
    作者:  Matthew SwireCharles ffrench-Constant, 日期: 10/20/2020, 浏览量: 3024, Q&A: 0
    Oligodendrocytes generate distinct patterns of myelination throughout the CNS. Variations in myelination along axons may enable neurons to fine-tune conduction velocities and alter signal synchronisation. Here we outline a staining protocol ...
    Double Labeling of PDGFR-β and α-SMA in Swine Models of Acute Kidney Injury to Detect Pericyte-to-Myofibroblast Transdifferentation as Early Marker of Fibrosis
    双标记PDGFR-β和α-SMA在猪急性肾损伤模型中检测周细胞-肌成纤维细胞转化作为早期纤维化标志物的研究
    Growing evidences suggest that peritubular capillaries pericytes are the main source of scar-forming myofibroblasts during chronic kidney disease (CKD), as well as early phases of acute kidney injury (AKI). In a swine model of sepsis and I/R ...
    The Chick Chorioallantoic Membrane (CAM) Assay as a Three-dimensional Model to Study Autophagy in Cancer Cells
    以鸡胚绒毛尿囊膜(CAM)为三维模型研究癌细胞自噬作用
    作者:  Félice A. Janser, Patricia Ney, Marta Teixeira Pinto, Rupert LangerMario P. Tschan, 日期: 07/05/2019, 浏览量: 7662, Q&A: 0
    The chick chorioallantoic membrane (CAM) is an extra-embryonic organ and thus well accessible for seeding and harvesting 3D cell cultures. Samples from CAM assays are suitable for protein and gene expression analysis as well as for ...
    Detection of mRNA by Whole Mount in situ Hybridization and DNA Extraction for Genotyping of Zebrafish Embryos
    整体原位杂交检测mRNA并提取DNA检测斑马鱼胚胎的基因分型
    作者:  Rachna NarayananAndrew C. Oates, 日期: 03/20/2019, 浏览量: 12156, Q&A: 0
    In situ hybridization is used to visualize the spatial distribution of gene transcripts in tissues and in embryos, providing important information about disease and development. Current methods involve the use of complementary riboprobes ...
    An Improved Staining Method for Low Signal LacZ Reporter Detection in Mouse Embryos
    一种改进的可用于检测小鼠胚胎弱 LacZ 报告信号的染色法
    作者:  Xiaopeng Shen, Feng Xu, Shen Wu, Meng Li, Jingyi Zhang, Rui LiangYu Liu, 日期: 03/05/2019, 浏览量: 5549, Q&A: 0
    In many fields of biology, especially in the field of developmental biology, LacZ reporter staining is an approach used to monitor gene expression patterns. In the LacZ reporter system, the LacZ gene is inserted in the ...
    Immunohistochemical Staining of TLR4 in Human Skeletal Muscle Samples
    人类骨骼肌样本中TLR4的免疫组化染色
    作者:  Daniela Verzola, Samantha Milanesi, Francesca CostiglioloGiacomo Garibotto, 日期: 01/20/2019, 浏览量: 4795, Q&A: 0
    Growing evidence suggests the involvement of TLR4, a receptor in the innate immune system, in muscle loss in uremia. Recently, we have evaluated TLR4 in human skeletal muscle from chronic kidney disease patients, by immunohistochemistry and image ...
    Mouse Mammary Gland Whole Mount Preparation and Analysis
    小鼠乳腺的全胚胎准备和分析
    作者:  Cornelia Tolg, Mary CowmanEva A. Turley, 日期: 07/05/2018, 浏览量: 14255, Q&A: 0
    The mammary gland undergoes extensive remodeling during pregnancy and is also subject to neoplastic processes both of which result in histological changes of the gland epithelial structure. Since the mammary tree is a complex three-dimensional ...