免疫学

分类

    Human Auto-IgG Purification from High Volume Serum Sample by Protein G Affinity Purification
    用蛋白G亲和纯化法从大量血清样品中纯化人类自身 IgG
    作者:  Serena SensiAndreas Goebel, 日期: 12/05/2022, 浏览量: 254, Q&A: 0

    Immunoglobulins are proteins produced by the immune system, which bind specifically to the antigen that induced their formation and target it for destruction. Highly purified human immunoglobulins are commonly used in research laboratories for

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    Identification of SARS-CoV-2 Neutralizing Antibody with Pseudotyped Virus-based Test on HEK-293T hACE2 Cells
    基于 HEK-293T hACE2 细胞的假型病毒试验鉴定 SARS-CoV-2 中和抗体
    作者:  Constant Gillot, Julien Favresse, Vincent Maloteau, Jean-Michel DognéJonathan Douxfils, 日期: 04/05/2022, 浏览量: 1136, Q&A: 0

    Neutralizing antibodies (NAbs) are of particular importance because they can prevent binding of the receptor binding domain (RBD) of the spike protein (S protein) to the angiotensin-converting enzyme 2 (ACE2) receptor present at the surface of

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    A High-throughput Automated ELISA Assay for Detection of IgG Antibodies to the SARS-CoV-2 Spike Protein
    一种高通量自动ELISA检测SARS-CoV-2刺突蛋白IgG抗体的方法

    The SARS-CoV-2 pandemic and vaccination campaign has illustrated the need for high throughput serological assays to quantitatively measure antibody levels. Here, we present a protocol for a high-throughput colorimetric ELISA assay to detect IgG

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    Production of Recombinant Replication-defective Lentiviruses Bearing the SARS-CoV or SARS-CoV-2 Attachment Spike Glycoprotein and Their Application in Receptor Tropism and Neutralisation Assays
    携带 SARS-CoV 或 SARS-CoV-2 附着尖峰糖蛋白的重组复制缺陷慢病毒的生产及其在受体向性和中和试验中的应用
    作者:  Nazia Thakur, Giulia Gallo, Ahmed M. E. ElreafeyDalan Bailey, 日期: 11/05/2021, 浏览量: 2310, Q&A: 0

    For enveloped viruses, such as SARS-CoV-2, transmission relies on the binding of viral glycoproteins to cellular receptors. Conventionally, this process is recapitulated in the lab by infection of cells with isolated live virus. However, such

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    Production of the Receptor-binding Domain of the Viral Spike Proteins from 2003 and 2019 SARS CoVs and the Four Common Human Coronaviruses for Serologic Assays and Inhibitor Screening
    从2003年和2019年SARS冠状病毒和四种常见人类冠状病毒的血清学分析和抑制剂筛选中产生病毒刺突蛋白受体结合域

    The recombinant receptor-binding domain (RBD) of the viral spike protein from SARS-CoV-1 and 2 are reliable antigens for detecting viral-specific antibodies in humans. We and others have shown that the levels of RBD-binding antibodies and SARS-CoV-2

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    抗体滴定的流式检测
    Antibody Titration by Flow Cytometry
    作者:  刘懿蕃, 华兆琳侯百东, 日期: 11/20/2019, 浏览量: 5889, Q&A: 1
    摘要: ...
    Quantification of Serum Ovalbumin-specific Immunoglobulin E Titre via in vivo Passive Cutaneous Anaphylaxis Assay
    通过体内被动皮肤过敏试验定量血清卵清蛋白特异性免疫球蛋白E滴度
    Murine models of allergic airway disease are frequently used as a tool to elucidate the cellular and molecular mechanisms of tissue-specific asthmatic disease pathogenesis. Paramount to the success of these models is the induction of experimental ...
    Characterization of Mouse Adult Testicular Macrophage Populations by Immunofluorescence Imaging and Flow Cytometry
    免疫荧光成像和流式细胞术鉴定小鼠成人睾丸巨噬细胞群体
    作者:  Noushin Mossadegh-KellerMichael H. Sieweke, 日期: 03/05/2019, 浏览量: 5286, Q&A: 0
    Testicular macrophages (tMΦ) are the most abundant immune cells residing in the testis, an immune-privileged organ. TMΦ are known to exhibit different functions, such as protecting spermatozoa from auto-immune attack by producing immunosuppressive ...
    DNA Immunization Using in vivo Electroporation for Generating Monoclonal Antibodies Against Mouse IL-9R
    利用体内电穿孔产生小鼠IL-9R单克隆抗体进行基因免疫
    作者:  Shogo Takatsuka, Hiroyuki Yamada, Kei Haniuda, Marina Ichihashi, Joe ChibaDaisuke Kitamura, 日期: 02/20/2019, 浏览量: 5433, Q&A: 0
    Membrane proteins such as cytokine receptors and G protein-coupled receptors can be drug targets. Recently, we have generated specific monoclonal antibodies (mAbs) against the mouse IL-9 receptor (IL-9R) and found that IL-9R on memory B cells have ...