分子生物学

分类

    Conditional Human BRD4 Knock-In Transgenic Mouse Genotyping and Protein Isoform Detection
    条件性人 BRD4 敲入转基因小鼠基因分型和蛋白质异构体检测
    作者:  Michael Paul Lewis, Shwu-Yuan WuCheng-Ming Chiang, 日期: 04/05/2022, 浏览量: 1149, Q&A: 0

    Bromodomain-containing protein 4 (BRD4) is an acetyl-lysine reader protein and transcriptional regulator implicated in chromatin dynamics and cancer development. Several BRD4 isoforms have been detected in humans with the long isoform (BRD4-L,

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    Reference-free Association Mapping from Sequencing Reads Using k-mers
    利用k-mers进行测序读取的无参考关联映射
    作者:  Zakaria Mehrab, Jaiaid Mobin, Ibrahim Asadullah Tahmid, Lior PachterAtif Rahman, 日期: 11/05/2020, 浏览量: 4093, Q&A: 0
    Association mapping is the process of linking phenotypes with genotypes. In genome wide association studies (GWAS), individuals are first genotyped using microarrays or by aligning sequenced reads to reference genomes. However, both these approaches ...
    Methylation-sensitive Amplified Polymorphism as a Tool to Analyze Wild Potato Hybrids
    利用甲基化敏感扩增多态性分析野生马铃薯杂交种
    作者:  Nicolás Cara, Carlos F. Marfil, María V. BertoldiRicardo W. Masuelli, 日期: 07/05/2020, 浏览量: 3518, Q&A: 0
    Methylation-Sensitive Amplification Polymorphism (MSAP) is a versatile marker for analyzing DNA methylation patterns in non-model species. The implementation of this technique does not require a reference genome and makes it possible to determine ...
    Using indCAPS to Detect CRISPR/Cas9 Induced Mutations
    利用indCAPS检测CRISPR/Cas9诱导的突变
    作者:  Charles Hodgens, Zachary L. NimchukJoseph J. Kieber, 日期: 09/20/2019, 浏览量: 3094, Q&A: 0
    Cleaved amplified polymorphic sequences (CAPS) assays are useful tools for detecting small mutations such as single nucleotide polymorphisms (SNPs) or insertion/deletions (indels) present in an amplified DNA fragment. A mutation that disrupts or ...
    A High-throughput qPCR-based Method to Genotype the SOD1G93A Mouse Model for Relative Copy Number
    一种基于qPCR 的高通量研究 SOD1G93A小鼠模型中相对拷贝数的基因分型方法
    作者:  Valerie R. TassinariFernando G. Vieira, 日期: 06/20/2019, 浏览量: 5582, Q&A: 0
    The most commonly used mouse model in ALS preclinical research expresses multiple copies of the human SOD1 (G93A) transgene. During the course of breeding, successive generations of mice can lose copies of the transgene. Because shorter lifespan of ...
    Southern印记杂交
    Southern Blot
    作者:  吴昊, 陈太钰, 林拥军陈浩, 日期: 06/12/2018, 浏览量: 7362, Q&A: 0
    实验原理:Southern印记杂交 (Southern Blotting) 是将植物基因组总DNA酶切,通过琼脂糖凝胶电泳分离,变性之后利用虹吸原理印记到固相支持物上 ...
    水稻转基因材料培育的一般步骤
    General Steps for the Cultivation of Transgenic Rice Materials
    作者:  陈太钰, 吴昊, 林拥军陈浩, 日期: 06/12/2018, 浏览量: 6315, Q&A: 0
    实验原理:采用农杆菌介导的遗传转化获得转基因植株,经分子鉴定,表型检测,田间农艺性状考查以及安全评价后培育转基因水稻。
    实验目的:以野生型水稻品种为受体培育具有商业化前景的转基因水稻新材料。
    柑橘SSR分子标记操作方法
    SSR Polymorphism Analysis in Citrus
    作者:  余惠文, 程运江, 徐强邓秀新, 日期: 06/12/2018, 浏览量: 4538, Q&A: 0
    实验原理:聚丙烯酰胺凝胶具有分子筛效应,且分辨率极高。聚丙烯酰胺凝胶电泳(Polyacrylamide gel electrophoresis,PAGE)可以区分所扩增SSR片段间的差异,通过银染显色可以确认核酸中特异SSR的多态性。 ...
    水稻常用分子标记(SSR InDel SNP)检测
    Detection of Commonly-used Molecular Markers (SSR InDel SNP) in Rice
    作者:  孙文强, 申国境余四斌, 日期: 05/10/2018, 浏览量: 6260, Q&A: 0
    实验原理:分子标记(Molecular Markers)是以个体间核苷酸序列变异为基础的遗传标记,是DNA水平遗传多态性的直接反映。
           简单序列重复(Simple Sequence ...