植物科学

分类

    Application of Cadaverine to Inhibit Biotin Biosynthesis in Plants
    尸胺在抑制植物生物素合成中的应用
    作者:  Nicole M. Gibbs, Shih-Heng SuPatrick H. Masson, 日期: 04/20/2022, 浏览量: 1004, Q&A: 0

    Biotin is an essential vitamin in plants. However, characterization of biotin deficiency has been limited by embryo lethality in mutants, which can only be rescued by supplementation of biotin. Here, we describe a protocol to characterize

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    Split-luciferase Complementation Imaging Assay to Study Protein-protein Interactions in Nicotiana benthamiana
    分裂荧光素酶互补成像分析研究本氏烟草中的蛋白质之间相互作用
    作者:  Liping Wang, Gang Yu, Alberto P. MachoRosa Lozano-Durán, 日期: 12/05/2021, 浏览量: 3675, Q&A: 0

    The experimental identification of protein-protein interactions (PPIs) is critical to understand protein function. Thus, a plethora of sensitive and versatile approaches have been developed to detect PPIs in vitro or in vivo, such as protein

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    A Fast and Easy Method to Study Ralstonia solanacearum Virulence upon Transient Gene Expression or Gene Silencing in Nicotiana benthamiana Leaves
    利用本生烟叶片基因瞬时表达或沉默研究青枯菌毒力的快速简便方法
    作者:  Wenjia YuAlberto P. Macho, 日期: 08/05/2021, 浏览量: 2641, Q&A: 0

    Ralstonia solanacearum is a devastating soil-borne bacterial pathogen that causes disease in multiple host plants worldwide. Typical assays to measure virulence of R. solanacearum in laboratory conditions rely on soil-drenching inoculation followed

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    Chromatin Immunoprecipitation (ChIP) to Assess Histone Marks in Auxin-treated Arabidopsis thaliana Inflorescence Tissue
    染色质免疫沉淀法(ChIP)检测生长素处理拟南芥花序组织中的组蛋白标记
    作者:  André KuhnLars Østergaard, 日期: 12/05/2020, 浏览量: 2907, Q&A: 0
    Chromatin immunoprecipitation coupled with quantitative PCR (ChIP-qPCR) or high-throughput sequencing (ChIP-seq) has become the gold standard for the identification of binding sites of DNA binding proteins and the localization of histone ...
    Ratiometric Measurement of Protein Abundance after Transient Expression of a Transgene in Nicotiana benthamiana
    转基因烟草瞬时表达后蛋白质丰度的比例计量测定
    作者:  Aashima KhoslaDavid C. Nelson, 日期: 09/05/2020, 浏览量: 4029, Q&A: 0
    Ratiometric reporters are tools to dynamically measure the relative abundance of a protein of interest. In these systems, a target protein fused to a fluorescent or bioluminescent reporter is expressed with fixed stoichiometry to a reference protein ...
    Measuring Protein Half-life in Arabidopsis thaliana
    拟南芥蛋白半衰期的检测
    作者:  Hongqing GuoYanhai Yin, 日期: 08/05/2019, 浏览量: 4423, Q&A: 0
    Post-translational modifications play important roles in controlling protein function and can lead to altered protein stability. Protein stability can be determined after treatment with the protein synthesis inhibitor Cycloheximide. Cycloheximide is ...
    Fluorescence Immunolocalization in Mitotic Chromosome Spreads from Maize Embryos
    玉米胚中有丝分裂染色质的免疫荧光定位
    作者:  Jing-Han Liu, Ya-zhong Wang, Lin ChenYan He, 日期: 06/20/2019, 浏览量: 3189, Q&A: 0
    Immunolocalization is a necessary technique in the study of chromosome structure and function. It can provide important information for better understanding the relation between epigenetic modifications and chromosome condensation. In this protocol, ...
    Heat Induced Epitope Retrieval (HIER) Assisted Protein Immunostaining in Maize
    玉米中热诱导抗原修复辅助的蛋白免疫染色
    作者:  Katsutoshi TsudaGeorge Chuck, 日期: 06/05/2019, 浏览量: 3183, Q&A: 0
    Protein immunostaining provides important spatio-temporal information about gene expression. Even using high quality antibodies, signal reproducibility and specificity can be problematic depending on tissue fixation methods. For example, ...
    Protein Immunoprecipitation in Maize
    玉米蛋白质的免疫沉淀
    作者:  María Jazmín Abraham-Juárez, 日期: 06/05/2019, 浏览量: 3457, Q&A: 0
    Protein immunoprecipitation (IP) is a method used to identify cellular protein complexes. Whence, antibodies that specifically recognize a native bait protein or an epitope tag fused to the protein of interest allow the protein of interest to be ...
    Extraction and Purification of Laccases from Rice Stems
    水稻茎秆酶的提取与纯化
    作者:  Chenna SwethaP. V. Shivaprasad, 日期: 04/05/2019, 浏览量: 5108, Q&A: 0
    Laccases are found in cell walls of plants in very low amounts. This protocol provides an efficient method to purify laccases from rice stems. The method involves three steps: 1) Isolation of total protein from rice stems using buffers with high ...