植物科学

分类

    Faster Bacterial Gene Cloning Using the Brick into the Gateway (BiG) Protocol
    使用Brick into the Gateway (BiG) 实验方法快速克隆细菌基因

    Cloning systems like Gateway and Golden Gate/Braid are known because of their efficiency and accuracy. While the main drawback of Gateway is the expensive cost of the enzymes used in its two-step (LR and BP) reaction, Golden Gate requires

    ...
    Chromosome Scaffolding of Diploid Genomes Using ALLHiC
    使用ALLHiC构建二倍体基因组的染色体支架
    作者:  Yi-Bin WangXing-Tan Zhang, 日期: 09/20/2022, 浏览量: 167, Q&A: 0

    High-throughput chromosome conformation capture (Hi-C) technology has become an economical and robust tool for generating a chromosome-scale assembly. However, high-quality chromosome scaffoldings are limited by the number of short and chimeric

    ...
    The Canu Genome Assembly Pipeline Using Nanopore Long Reads
    使用 Nanopore Long Reads 的 Canu 基因组组装流程
    作者:  Guifang LinSanzhen Liu, 日期: 09/05/2022, 浏览量: 361, Q&A: 0

    Long sequencing reads have greatly improved assemblies of genomes with all sizes. The current Oxford Nanopore technology can regularly produce reads longer than 20 kb with less than 10% sequencing errors. To use long reads that contain relatively

    ...
    Investigation of Transposon DNA Methylation and Copy Number Variation in Plants Using Southern Hybridisation
    利用Southern杂交技术研究植物中转座子DNA甲基化和拷贝数变异的情况
    作者:  Vivek Hari Sundar G.P. V. Shivaprasad, 日期: 06/05/2022, 浏览量: 1139, Q&A: 0

    Plant genomes are pronouncedly enriched in repeat elements such as transposons. These repeats are epigenetically regulated by DNA methylation. Whole genome high-depth sequencing after bisulfite treatment remains an expensive and laborious method to

    ...
    Isolation of Plant Nuclei Compatible with Microfluidic Single-nucleus ATAC-sequencing
    与微流体单核 ATAC 测序兼容的植物核的分离
    作者:  Sandra B. Thibivilliers, Dirk K. AndersonMarc Y. Libault, 日期: 12/05/2021, 浏览量: 2455, Q&A: 0

    Gene expression depends on the binding of transcription factors with DNA regulatory sequences. The level of accessibility for these sequences varies between cells and cell types. Until recently, using the Tn5 assay for transposase-accessible

    ...
    Measurement of Transgene Copy Number in Plants Using Droplet Digital PCR
    应用微滴数字PCR技术测定植物中的转基因拷贝数
    作者:  Yao-Min Cai, Quentin M. DudleyNicola J. Patron, 日期: 07/05/2021, 浏览量: 3482, Q&A: 1

    Transgenic plants are produced both to investigate gene function and to confer desirable traits into crops. Transgene copy number is known to influence expression levels, and consequently, phenotypes. Similarly, knowledge of transgene zygosity is

    ...
    Cellulase and Macerozyme-PEG-mediated Transformation of Moss Protoplasts
    纤维素酶和离析酶-PEG介导的苔藓原生质体转化
    This protocol describes the generation of protoplasts from protonemal tissue of the moss Physcomitrium patens (syn. Physcomitrella patens), using Cellulase ONOZUKA R10 and Macerozyme R10, followed by polyethylene glycol (PEG) ...
    Efficient Transient Gene Knock-down in Tobacco Plants Using Carbon Nanocarriers
    应用碳纳米载体对烟草基因进行高效瞬时敲减
    作者:  Gozde S. DemirerMarkita P. Landry, 日期: 01/05/2021, 浏览量: 4366, Q&A: 0
    Gene knock-down in plants is a useful approach to study genotype-phenotype relationships, render disease resistance to crops, and enable efficient biosynthesis of molecules in plants. Small interfering RNA (siRNA)-mediated gene silencing is one of ...
    Dual sgRNA-based Targeted Deletion of Large Genomic Regions and Isolation of Heritable Cas9-free Mutants in Arabidopsis
    基于双sgRNA的拟南芥大基因组区域靶向清除及可遗传的非Cas9突变体的分离
    作者:  Yu JinSebastian Marquardt, 日期: 10/20/2020, 浏览量: 4776, Q&A: 0
    CRISPR/Cas9 system directed by a gene-specific single guide RNA (sgRNA) is an effective tool for genome editing such as deletions of few bases in coding genes. However, targeted deletion of larger regions generate loss-of-function alleles ...
    Safe DNA-extraction Protocol Suitable for Studying Tree-fungus Interactions
    适于研究树-真菌互作的安全DNA提取方法
    作者:  Susanna Keriö, Eeva TerhonenJared M. LeBoldus, 日期: 06/05/2020, 浏览量: 5604, Q&A: 0
    We present a safe and low-cost method suitable for DNA extraction from mycelium and tree tissue samples. After sample preparation, the extraction takes about 60 min. Method performance was tested by extracting DNA from various tree tissue samples ...