植物科学

Cloning systems like Gateway and Golden Gate/Braid are known because of their efficiency and accuracy. While the main drawback of Gateway is the expensive cost of the enzymes used in its two-step (LR and BP) reaction, Golden Gate requires

High-throughput chromosome conformation capture (Hi-C) technology has become an economical and robust tool for generating a chromosome-scale assembly. However, high-quality chromosome scaffoldings are limited by the number of short and chimeric

Long sequencing reads have greatly improved assemblies of genomes with all sizes. The current Oxford Nanopore technology can regularly produce reads longer than 20 kb with less than 10% sequencing errors. To use long reads that contain relatively

Plant genomes are pronouncedly enriched in repeat elements such as transposons. These repeats are epigenetically regulated by DNA methylation. Whole genome high-depth sequencing after bisulfite treatment remains an expensive and laborious method to

Gene expression depends on the binding of transcription factors with DNA regulatory sequences. The level of accessibility for these sequences varies between cells and cell types. Until recently, using the Tn5 assay for transposase-accessible

Transgenic plants are produced both to investigate gene function and to confer desirable traits into crops. Transgene copy number is known to influence expression levels, and consequently, phenotypes. Similarly, knowledge of transgene zygosity is