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A high-quality database for basic life science protocols
实验方法
研究领域
癌症生物学 (380)
发育生物学 (258)
分子生物学 (926)
干细胞 (203)
免疫学 (521)
神经科学 (552)
生物化学 (1186)
生物物理学 (102)
微生物学 (1009)
系统生物学 (263)
细胞生物学 (1734)
植物科学 (930)
Bio-protocol 期刊
特刊
课题组实验方法合辑
Request a Protocol
提交
提交实验方法
作者指南
关于
癌症生物学
最新视频
Self-Paced Five-Choice Serial Reaction Time-Task for Mouse Behavioral Testing
分类
日期
浏览量
In vitro
STING Activation with the cGAMP-STINGΔTM Signaling Complex
cGAMP-STINGΔTM信号复合物体外激活STING
作者:
Yanpu He
,
Celestine Hong
,
Darrell J. Irvine
,
Jiahe Li
和
Paula T. Hammond
,
日期:
02/05/2021,
浏览量:
4004,
Q&A:
0
Activating the STING (stimulator of interferon genes) signaling pathway via administration of STING agonist cyclic GMP-AMP (cGAMP) has shown great promise in cancer immunotherapy. While state-of-the-art approaches have predominantly focused on the ...
肿瘤微环境内DC细胞亚型和成熟流式检测方法
The Detection of Dendritic Cell Subsets and Maturation in the Tumor Microenvironment by Flow Cytometry
作者:
杨超
,
金静思
和
邓刘福
,
日期:
11/20/2019,
浏览量:
5905,
Q&A:
0
摘要:
树突状细胞是一群功能强大的抗原提呈细胞,是联系固有免疫和特异性免疫的中坚力量。cDC表达CD11c和MHCII,可分为cDC1和cDC2两个不同的亚群 ...
肿瘤微环境内巨噬细胞极化的流式检测方法
The Detection of Macrophage Polarization in the Tumor Microenvironment by Flow Cytometry
作者:
金静思
,
杨超
和
邓刘福
,
日期:
11/20/2019,
浏览量:
9790,
Q&A:
0
摘要:
肿瘤的发生进展及治疗预后,与其赖以生存的肿瘤微环境息息相关。免疫细胞作为机体重要的监视防御力量,在肿瘤内部可能扮演着截然不同的角色。M2极化型的巨噬细胞呈现出免疫抑制状态,能够促进肿瘤发生,血管再生及转移 ...
微环境内MDSC亚群流式检测
The Detection of Myeloid Derived Suppressor Cells in the Tumor Microenvironment by Flow Cytometry
作者:
金静思
,
杨超
和
邓刘福
,
日期:
11/20/2019,
浏览量:
4283,
Q&A:
0
摘要:
在肿瘤免疫微环境中主要起着促肿瘤作用的髓系来源抑制细胞 (myeloid derived suppressor cells,MDSCs)可分为多形核髓系来源抑制细胞 (polymorphonucear MDSCs,PMN-MDSCs) 和单核髓系来源抑制细胞 ...
3D Co-culture System of Tumor-associated Macrophages and Ovarian Cancer Cells
肿瘤相关巨噬细胞和卵巢癌细胞的3D共培养系统
作者:
Lingli Long
,
Mingzhu Yin
和
Wang Min
,
日期:
04/20/2018,
浏览量:
8629,
Q&A:
0
Ovarian cancer is fairly unique in that ovarian carcinoma cells can detach and spread directly through peritoneal cavity. It has been unclear, however, how detached cancer cells survive in the peritoneum and form spheroid structure. We have recently ...
Peptide Loading on MHC Class I Molecules of Tumor Cells
肿瘤细胞表面MHC I类分子结合肽实验
作者:
Loredana Cifaldi
,
Franco Locatelli
和
Doriana Fruci
,
日期:
09/20/2016,
浏览量:
9832,
Q&A:
0
MHC class I molecules present peptides to cytotoxic T cells allowing the immune system to scan for intracellular pathogens and mutated proteins. The generation of antigenic peptides is a multistep process that ends in the endoplasmic reticulum (ER). ...
Killer Cell Ig-like Receptors (KIR)-Binding Assay for Tumor Cells
杀伤细胞免疫球蛋白样受体结合肿瘤细胞实验
作者:
Loredana Cifaldi
,
Franco Locatelli
和
Doriana Fruci
,
日期:
09/20/2016,
浏览量:
6369,
Q&A:
0
Natural killer (NK) cells play key roles in innate and adaptive immune responses against virus and tumor cells. Their function relies on the dynamic balance between activating and inhibiting signals through receptors that bind ligands expressed on ...
Differentiation of THP1 Cells into Macrophages for Transwell Co-culture Assay with Melanoma Cells
THP1细胞分化成巨噬细胞及与黑色素瘤细胞的跨室联合培养试验
作者:
Michael Peter Smith
,
Helen Young
,
Adam Hurlstone
和
Claudia Wellbrock
,
日期:
11/05/2015,
浏览量:
43689,
Q&A:
2
Understanding how immune cells such as macrophages interact with cancer cells is of increasing interest, as cancer treatments move towards combing both targeted- and immuno-therapies in new treatment regimes. This protocol is using THP-1 cells, a ...
In vitro
Regulatory T cells Differentiation From Naïve T Cells
从初始T细胞到调节性T细胞的体外分化实验
作者:
Tomás Dalotto-Moreno
,
Gabriel A. Rabinovich
和
Marian Salatino
,
日期:
03/20/2014,
浏览量:
21723,
Q&A:
1
In the past years, a subset of regulatory T cells (Tregs) expressing CD4, CD25 and the transcription factor FoxP3 has gained considerable attention as key regulators of T-cell tolerance and homeostasis (Sakaguchi, 2004). This population of T cells ...