酵母双杂交技术检测蛋白互作
Yeast Two-hybrid Assay for Detecting Protein Interaction
Authors: 毛文文 魏灵芝 ,
李冰冰 ,
贾文锁 and
date: 11/29/2018,
view: 8801,
Q&A: 1
材料与试剂枪头离心管冰ssDNA (10 mg/ml)质粒DNAAH109菌液酵母感受态DMSOPEG4000 (50%)10x LiAc (1 M)Tris-HClEDTA (pH7.5)YPD0.2% Ade琼脂粉SD-baseSD/-leu/-trpSD/-ade/-leu/-trp/-his10x TE (见溶液配方)1x TE/LiAc (见溶液配方)PEG/LiAc (见溶液配方)液体和固体YPDA培养基 (1 L) (见溶液配方)营养缺陷型培养板 (1 L) (见溶液配方)1)二缺培养板2)四缺培养板仪器设备三角瓶移液枪涡旋振荡器水浴锅分光光度计离心机摇床培养箱高压灭菌锅实验步骤