Standard PCR Protocol
标准PCR方案
Author: Fanglian Hedate: 04/05/2011,
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Q&A: 8
... This protocol describes basic steps of a PCR experiment using home-made Taq DNA polymerase. ... Temperature gradient PCR is often a way to finalize an optimal annealing temperatu
Single Worm PCR
单蠕虫PCR
Author: Fanglian Hedate: 04/20/2011,
view: 20975,
Q&A: 1
Also, try not to pick an old worm or starving worm.Spin capped PCR tubes briefly to bring down worm to Store the worm DNA at -80 °C (or -20 °C for temporary storage) if needed.Perform standar