Abstract
Neonatal mouse heart can regenerate after cardiac injury within postnatal 7 days, while adult mouse can not. Using the neonatal mouse myocardial infarction model to investigate the molecular mechanism of heart regeneration is important for finding new therapeutic strategies for myocardial infarction patients.
Equipment
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Equipment
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Company
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Identifier
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stereoscopic microscope
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Zeiss
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Stemi 305
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microforceps
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Shanghai Jinzhong
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WA3080
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microscissors
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Shanghai Jinzhong
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WAB040
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micro-needle holder
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Shanghai Jinzhong
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WA2050
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Notes
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Be careful to control the depth of anesthesia, too shallow anaesthesia is not conducive to operation, while excessive anesthesia can increase the mortality of mice after operation. The depth of anesthesia was determined by limb movement and breathing.
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Do not injure the lungs during operation.
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Operate gently to reduce bleeding.
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Do not let the silk sutures cut the myocardium at an angle when tying the knot, keep three points in line.
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The mice should be sent back to the mother’s cage in time after rewarming, and the mice could be wiped with the original padding in its mother’s cage if necessary.
[关键词] Myocardial infarction; heart regeneration; animal model; neonatal
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