Product Description
KAPA Library Amplification Kits for Illumina platforms are designed for the amplification of next-generation sequencing libraries prepared for Illumina sequencing.
In order to maximize sequence coverage uniformity, it is critical to minimize library amplification bias. KAPA HiFi DNA Polymerase is designed for low-bias, high-fidelity PCR, and is the reagent of choice for NGS library amplification1,2,3,4. KAPA Library Amplification Kits include KAPA HiFi HotStart ReadyMix (2X), a ready-touse PCR mix comprising all the components for library amplification— except primers and template. Kits also include Library Amplification Primer Mix (10X), designed for the high-efficiency amplification of Illumina libraries flanked by adapters containing the P5 and P7 flow cell sequences.
- Oyola, S.O., et al., BMC Genomics 13, 1 (2012).
- Quail, M.A., et al., Nature Methods 9, 10 (2012).
- Quail, M.A., et al., BMC Genomics 13, 341 (2012).
- Ross, M.G., et al., Genome Biology 14, R51 (2013).
Product Applications
KAPA Library Amplification Kits for Illumina platforms are suited for high-efficiency, high-fidelity, low-bias amplification of libraries prior to Illumina sequencing. This includes libraries prepared for:
• whole-genome shotgun sequencing
• targeted sequencing (pre- and post-capture amplification)
• amplicon sequencing
• ChIP-seq
• RNA-seq.
Library Amplification Protocol
Library Amplification
Library Amplification Primer Mix (10X) (KK2623), sold separately, is designed to eliminate or delay primer depletion during library amplification reactions performed with KAPA HiFi HotStart ReadyMix (2X). The primer mix is suitable for the amplification of all Illumina libraries flanked by the P5 and P7 flow cell sequences. Primers are supplied at a 10X concentration of 20 μM each, and have been formulated as previously described. User supplied primer mixes may be used in combination with incomplete or custom adapters. For guidelines on the formulation of user-supplied library amplification primers, please contact Technical Support at sequencing.roche.com/support.
Post-amplification Cleanup