Procedure
400 ng DNase-treated RNA and a total of 9 cycles of PCR amplification of the library. The libraries were prepared using the KAPA RNA HyperPrep Kit with RiboErase (HMR) (Kapa Biosystems). We followed the step-by-step protocol from the manufacturer (see Reference 2), attached using 400 ng DNase-treated RNA and a total of 9 cycles of PCR amplification of the library.
**DO NOT OVERDRY.**Proceed immediately to next step.
References
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