Bio-101 - Improve Research Reproducibility

Fluorescence activated cell sorting in Schistosoma mansoni

Author: George R Wendt
Date: Apr 28, 2020，Views：1105

Original protocol used in research article：

Flatworm-specific transcriptional regulators promote the specification of tegumental progenitors in Schistosoma mansoni
eLife, Mar 20, 2018; DOI: 10.7554/eLife.33221

Bioinformatic analysis of the ChIP-seq and CUT&RUN datasets

Authors: Huacheng Luo and Jianrong Lu
Date: Apr 25, 2020，Views：1492

Original protocol used in research article：

LATS kinase–mediated CTCF phosphorylation and selective loss of genomic binding
Science Advances, Feb 19, 2020; DOI: 10.1126/sciadv.aaw4651

Chromatin immunoprecipitation (ChIP) assay

Authors: Shuchi Agrawal Singh and Klaus Hansen
Date: Apr 24, 2020，Views：2557

Original protocol used in research article：

PLZF targets developmental enhancers for activation during osteogenic differentiation of human mesenchymal stem cells
eLife, Jan 23, 2019; DOI: 10.7554/eLife.40364

Preparation and use of stimulation beads

Authors: Michael C. Milone and Benjamin I Philipson
Date: Apr 23, 2020，Views：1061

Original protocol used in research article：

4-1BB costimulation promotes CAR T cell survival through noncanonical NF-κB signaling
Science Signaling, Mar 30, 2020; DOI: 10.1126/scisignal.aay8248

Protein expression

Author: Caileen M Brison
Date: Apr 22, 2020，Views：1177

Original protocol used in research article：

An order-to-disorder structural switch activates the FoxM1 transcription factor
eLife, May 28, 2019; DOI: 10.7554/eLife.46131

Aversive phototaxic suppression (APS)

Authors: Chunghun Lim and Yoonhee Ki
Date: Apr 21, 2020，Views：1688

Original protocol used in research article：

Sleep-promoting effects of threonine link amino acid metabolism in Drosophila neuron to GABAergic control of sleep drive
eLife, Jul 17, 2019; DOI: 10.7554/eLife.40593

Generation and LED light photoactivation of Opto-receptors

Authors: Anassuya Ramachandran and Caroline S Hill
Date: Apr 20, 2020，Views：1113

Original protocol used in research article：

TGF-β uses a novel mode of receptor activation to phosphorylate SMAD1/5 and induce epithelial-to-mesenchymal transition
eLife, Jan 29, 2018; DOI: 10.7554/eLife.31756

Magnet-assisted flow cytometry of in vivo tumors to quantitate cell-specific responses to magnetic iron oxide nanoparticles

Authors: Preethi Korangath and Robert Ivkov
Date: Apr 17, 2020，Views：1072

Original protocol used in research article：

Nanoparticle interactions with immune cells dominate tumor retention and induce T cell–mediated tumor suppression in models of breast cancer
Science Advances, Mar 24, 2020; DOI: 10.1126/sciadv.aay1601

RNA seq data analysis

Author: Ilaria Granata
Date: Apr 15, 2020，Views：1088

Original protocol used in research article：

Human axial progenitors generate trunk neural crest cells in vitro
eLife, Aug 10, 2018; DOI: 10.7554/eLife.35786

Immunofluorescent staining of mouse embryonic tissues

Authors: Jingyue Xu and Rulang Jiang
Date: Apr 14, 2020，Views：970

Original protocol used in research article：

Hedgehog signaling patterns the oral-aboral axis of the mandibular arch
eLife, Jan 14, 2019; DOI: 10.7554/eLife.40315

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