Bio-101 - Improve Research Reproducibility

Purification of APP C100-FLAG substrate

Authors: David M Bolduc, Michael S Wolfe and Dennis J Selkoe
Date: Apr 14, 2020，Views：1171

Original protocol used in research article：

The amyloid-beta forming tripeptide cleavage mechanism of γ-secretase
eLife, Aug 31, 2016; DOI: 10.7554/eLife.17578

Step-by-step lamina propria cell isolation protocol for H. polygyrus infected intestines

Author: Johannes U Mayer
Date: Apr 13, 2020，Views：1373

Original protocol used in research article：

High-dimensional analysis of intestinal immune cells during helminth infection
eLife, Feb 11, 2020; DOI: 10.7554/eLife.51678

β-Arrestin recruitment

Authors: Alix A. J. Rouault and Julien A. Sebag
Date: Apr 13, 2020，Views：1107

Original protocol used in research article：

The GPCR accessory protein MRAP2 regulates both biased signaling and constitutive activity of the ghrelin receptor GHSR1a
Science Signaling, Jan 6, 2020; DOI: 10.1126/scisignal.aax4569

Whole mount immunostaining

Author: Manju Sharma
Date: Apr 13, 2020，Views：1233

Original protocol used in research article：

Identification of EOMES-expressing spermatogonial stem cells and their regulation by PLZF
eLife, May 31, 2019; DOI: 10.7554/eLife.43352

Cell surface ELISA

Author: Amanda N Hayward
Date: Apr 13, 2020，Views：1398

Original protocol used in research article：

A toolkit for studying cell surface shedding of diverse transmembrane receptors
eLife, Jun 7, 2019; DOI: 10.7554/eLife.46983

RNP assembly for HDR reaction

Authors: Xinyu Ling and Tao Liu
Date: Apr 13, 2020，Views：1192

Original protocol used in research article：

Improving the efficiency of precise genome editing with site-specific Cas9-oligonucleotide conjugates
Science Advances, Apr 7, 2020; DOI: 10.1126/sciadv.aaz0051

Expression and purification of azido-Cas9 mutants

Authors: Xinyu Ling and Tao Liu
Date: Apr 13, 2020，Views：1237

Original protocol used in research article：

Improving the efficiency of precise genome editing with site-specific Cas9-oligonucleotide conjugates
Science Advances, Apr 7, 2020; DOI: 10.1126/sciadv.aaz0051

Bacterial culturing

Authors: Lina Yao and A Sloan Devlin
Date: Apr 11, 2020，Views：1127

Original protocol used in research article：

A selective gut bacterial bile salt hydrolase alters host metabolism
eLife, Jul 17, 2018; DOI: 10.7554/eLife.37182

Docking, pharmacophore modeling, virtual screening, and selection of test compounds

Authors: Teresa Kaserer and Daniela Schuster
Date: Apr 11, 2020，Views：1672

Original protocol used in research article：

Modulating FOXO3 transcriptional activity by small, DBD-binding molecules
eLife, Dec 4, 2019; DOI: 10.7554/eLife.48876

Identification of methylation markers discriminating between CRC and normal blood

Authors: Huiyan Luo and Rui-hua Xu
Date: Apr 10, 2020，Views：961

Original protocol used in research article：

Circulating tumor DNA methylation profiles enable early diagnosis prognosis prediction and screening for colorectal cancer
Science Translational Medicine, Dec 31, 2019; DOI: 10.1126/scitranslmed.aax7533

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