Bio-101 - Improve Research Reproducibility

Assessment of mortality

Author: Xia Li
Date: Mar 4, 2020，Views：1255

Original protocol used in research article：

Longitudinal trajectories, correlations and mortality associations of nine biological ages across 20-years follow-up
eLife, Feb 11, 2020; DOI: 10.7554/eLife.51507

Fly strains

Authors: Fengwei Yu and menglong Rui
Date: Mar 3, 2020，Views：1076

Original protocol used in research article：

Patronin governs minus-end-out orientation of dendritic microtubules to promote dendrite pruning in Drosophila
eLife, Mar 28, 2019; DOI: 10.7554/eLife.39964

Genome editing by CRISPR-Cas9

Authors: Rui Jia and Juan S Bonifacino
Date: Mar 2, 2020，Views：1189

Original protocol used in research article：

Negative regulation of autophagy by UBA6-BIRC6–mediated ubiquitination of LC3
eLife, Nov 6, 2019; DOI: 10.7554/eLife.50034

Construction of the RapiDeg yeast strain

Author: Lu Zhu
Date: Feb 26, 2020，Views：1120

Original protocol used in research article：

ESCRTs function directly on the lysosome membrane to downregulate ubiquitinated lysosomal membrane proteins
eLife, Jun 29, 2017; DOI: 10.7554/eLife.26403

Bayesian inference of progenitor types

Authors: Alfredo Llorca and Oscar Marin
Date: Feb 26, 2020，Views：1078

Original protocol used in research article：

A stochastic framework of neurogenesis underlies the assembly of neocortical cytoarchitecture
eLife, Nov 18, 2019; DOI: 10.7554/eLife.51381

Colony formation assay

Author: Ralph Patrick
Date: Feb 25, 2020，Views：4248

Original protocol used in research article：

Single-cell expression profiling reveals dynamic flux of cardiac stromal, vascular and immune cells in health and injury
eLife, Mar 26, 2019; DOI: 10.7554/eLife.43882

Genome Editing

Author: Grant Parker Flowers
Date: Feb 25, 2020，Views：1192

Original protocol used in research article：

Multiplex CRISPR/Cas screen in regenerating haploid limbs of chimeric Axolotls
eLife, Jan 28, 2020; DOI: 10.7554/eLife.48511

Single cell isolation from SVF

Author: Prashant Rajbhandari
Date: Feb 25, 2020，Views：1415

Original protocol used in research article：

Single cell analysis reveals immune cell–adipocyte crosstalk regulating the transcription of thermogenic adipocytes
eLife, Oct 23, 2019; DOI: 10.7554/eLife.49501

Spike phase-coupling code

Authors: Hugo Balleza-Tapia and André Fisahn
Date: Feb 25, 2020，Views：1149

Original protocol used in research article：

TrpV1 receptor activation rescues neuronal function and network gamma oscillations from Aβ-induced impairment in mouse hippocampus in vitro
eLife, Nov 12, 2018; DOI: 10.7554/eLife.37703

Adipocyte nuclei isolation from iWAT

Author: Prashant Rajbhandari
Date: Feb 24, 2020，Views：2050

Original protocol used in research article：

Single cell analysis reveals immune cell–adipocyte crosstalk regulating the transcription of thermogenic adipocytes
eLife, Oct 23, 2019; DOI: 10.7554/eLife.49501

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