Bio-101 - Improve Research Reproducibility

Observation and motility assay of zebrafish spermatozoa

Authors: Masahide Kikkawa and Hiroshi Yamaguchi
Date: Nov 18, 2019，Views：1238

Original protocol used in research article：

Systematic studies of all PIH proteins in zebrafish reveal their distinct roles in axonemal dynein assembly
eLife, May 9, 2018; DOI: 10.7554/eLife.36979

Western blots

Author: Joel Hrit
Date: Nov 18, 2019，Views：1100

Original protocol used in research article：

OGT binds a conserved C-terminal domain of TET1 to regulate TET1 activity and function in development
eLife, Oct 16, 2018; DOI: 10.7554/eLife.34870

4TU metabolic labeling and RNA analysis

Author: Leon Chan
Date: Nov 18, 2019，Views：1463

Original protocol used in research article：

Non-invasive measurement of mRNA decay reveals translation initiation as the major determinant of mRNA stability
eLife, Sep 7, 2018; DOI: 10.7554/eLife.32536

Immunofluorescence assay for 5hmc

Author: Vladimir Camarena
Date: Nov 18, 2019，Views：1372

Original protocol used in research article：

cAMP signaling regulates DNA hydroxymethylation by augmenting the intracellular labile ferrous iron pool
eLife, Dec 14, 2017; DOI: 10.7554/eLife.29750

GFP dropout screening

Author: Jason Sheltzer
Date: Nov 18, 2019，Views：1414

Original protocol used in research article：

CRISPR/Cas9 mutagenesis invalidates a putative cancer dependency targeted in on-going clinical trials
eLife, Mar 24, 2017; DOI: 10.7554/eLife.24179

Rotarod (Ugo Basile Biological Research Apparatus)

Author: Cristian A Lasagna-Reeves
Date: Nov 18, 2019，Views：1974

Original protocol used in research article：

Ataxin-1 oligomers induce local spread of pathology and decreasing them by passive immunization slows Spinocerebellar ataxia type 1 phenotypes
eLife, Dec 17, 2015; DOI: 10.7554/eLife.10891

Detailed protocol for mouse adipose tissue protein extraction

Authors: Yu An and Philipp E Scherer
Date: Nov 18, 2019，Views：4392

Original protocol used in research article：

Angiopoietin-2 in white adipose tissue improves metabolic homeostasis through enhanced angiogenesis
eLife, Mar 29, 2017; DOI: 10.7554/eLife.24071

Native-PAGE to study BiP oligomeric complexes

Author: Steffen Preissler
Date: Nov 18, 2019，Views：6639

Original protocol used in research article：

AMPylation matches BiP activity to client protein load in the endoplasmic reticulum
eLife, Dec 17, 2015; DOI: 10.7554/eLife.12621

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