Bio-101 - Improve Research Reproducibility

Immunofluorescence and imaging

Author: Aimin Peng
Date: Feb 18, 2020，Views：1082

Original protocol used in research article：

Kinesin Kif2C in regulation of DNA double strand break dynamics and repair
eLife, Jan 17, 2020; DOI: 10.7554/eLife.53402

Tissue processing for small capillary immunochemistry

Authors: Guillermo Luxán, Mara E Pitulescu and Ralf H Adams
Date: Feb 18, 2020，Views：1199

Original protocol used in research article：

Endothelial EphB4 maintains vascular integrity and transport function in adult heart
eLife, Nov 29, 2019; DOI: 10.7554/eLife.45863

Trypsin digestion

Author: Giulia Bertolin
Date: Feb 17, 2020，Views：1617

Original protocol used in research article：

Aurora kinase A localises to mitochondria to control organelle dynamics and energy production
eLife, Aug 2, 2018; DOI: 10.7554/eLife.38111

Cell culture

Author: Gabriela Dankova
Date: Feb 13, 2020，Views：1235

Original protocol used in research article：

Novel genetic loci affecting facial shape variation in humans
eLife, Nov 26, 2019; DOI: 10.7554/eLife.49898

Radiolabelled nutrient uptake for suspension cells

Author: Linda V Sinclair
Date: Feb 12, 2020，Views：1136

Original protocol used in research article：

Antigen receptor control of methionine metabolism in T cells
eLife, Mar 27, 2019; DOI: 10.7554/eLife.44210

Single-cell transcriptomics platform: 10x Chromium

Authors: Ralph Patrick, Robert E Nordon and Richard P Harvey
Date: Feb 11, 2020，Views：1710

Original protocol used in research article：

Single-cell expression profiling reveals dynamic flux of cardiac stromal, vascular and immune cells in health and injury
eLife, Mar 26, 2019; DOI: 10.7554/eLife.43882

Mouse tracking

Authors: Yair Shemesh, Oren Forkosh, Alon Chen and Elad Schneidman
Date: Feb 2, 2020，Views：1652

Original protocol used in research article：

High-order social interactions in groups of mice
eLife, Sep 3, 2013; DOI: 10.7554/eLife.00759

Calculation of tunnel volume

Authors: Fatima Alejandra Pardo Avila, Renuka Kudva and Gunnar von Heijne
Date: Jan 30, 2020，Views：1773

Original protocol used in research article：

The shape of the bacterial ribosome exit tunnel affects cotranslational protein folding
eLife, Nov 26, 2018; DOI: 10.7554/eLife.36326

Detailed BrdU immuno protocol with anti-YFP pre-staining

Authors: Zoe Christenson Wick, Esther Krook-Magnuson and Madison Tetzlaff
Date: Jan 27, 2020，Views：1029

Original protocol used in research article：

Novel long-range inhibitory nNOS-expressing hippocampal cells
eLife, Oct 14, 2019; DOI: 10.7554/eLife.46816

Pull-down and identification of the ligand

Author: Timo Vögtle
Date: Jan 23, 2020，Views：1254

Original protocol used in research article：

Heparan sulfates are critical regulators of the inhibitory megakaryocyte-platelet receptor G6b-B
eLife, Aug 22, 2019; DOI: 10.7554/eLife.46840

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