Protocols shared by authors via RaP service
Journal
Immunofluorescence and imaging
Author: Aimin Peng
Date: Feb 18, 2020，Views：1237
Original protocol used in research article：
Kinesin Kif2C in regulation of DNA double strand break dynamics and repair
eLife, Jan 17, 2020; DOI: 10.7554/eLife.53402
Tissue processing for small capillary immunochemistry
Authors: Guillermo Luxán, Mara E Pitulescu and Ralf H Adams
Date: Feb 18, 2020，Views：1375
Original protocol used in research article：
Endothelial EphB4 maintains vascular integrity and transport function in adult heart
eLife, Nov 29, 2019; DOI: 10.7554/eLife.45863
Trypsin digestion
Author: Giulia Bertolin
Date: Feb 17, 2020，Views：1865
Original protocol used in research article：
Aurora kinase A localises to mitochondria to control organelle dynamics and energy production
eLife, Aug 2, 2018; DOI: 10.7554/eLife.38111
Cell culture
Author: Gabriela Dankova
Date: Feb 13, 2020，Views：1422
Original protocol used in research article：
Novel genetic loci affecting facial shape variation in humans
eLife, Nov 26, 2019; DOI: 10.7554/eLife.49898
Radiolabelled nutrient uptake for suspension cells
Author: Linda V Sinclair
Date: Feb 12, 2020，Views：1371
Original protocol used in research article：
Antigen receptor control of methionine metabolism in T cells
eLife, Mar 27, 2019; DOI: 10.7554/eLife.44210
Single-cell transcriptomics platform: 10x Chromium
Authors: Ralph Patrick, Robert E Nordon and Richard P Harvey
Date: Feb 11, 2020，Views：1889
Original protocol used in research article：
Single-cell expression profiling reveals dynamic flux of cardiac stromal, vascular and immune cells in health and injury
eLife, Mar 26, 2019; DOI: 10.7554/eLife.43882
Mouse tracking
Authors: Yair Shemesh, Oren Forkosh, Alon Chen and Elad Schneidman
Date: Feb 2, 2020，Views：1904
Original protocol used in research article：
High-order social interactions in groups of mice
eLife, Sep 3, 2013; DOI: 10.7554/eLife.00759
Calculation of tunnel volume
Authors: Fatima Alejandra Pardo Avila, Renuka Kudva and Gunnar von Heijne
Date: Jan 30, 2020，Views：1997
Original protocol used in research article：
The shape of the bacterial ribosome exit tunnel affects cotranslational protein folding
eLife, Nov 26, 2018; DOI: 10.7554/eLife.36326
Detailed BrdU immuno protocol with anti-YFP pre-staining
Authors: Zoe Christenson Wick, Esther Krook-Magnuson and Madison Tetzlaff
Date: Jan 27, 2020，Views：1196
Original protocol used in research article：
Novel long-range inhibitory nNOS-expressing hippocampal cells
eLife, Oct 14, 2019; DOI: 10.7554/eLife.46816
Pull-down and identification of the ligand
Author: Timo Vögtle
Date: Jan 23, 2020，Views：1410
Original protocol used in research article：
Heparan sulfates are critical regulators of the inhibitory megakaryocyte-platelet receptor G6b-B
eLife, Aug 22, 2019; DOI: 10.7554/eLife.46840