Bio-101 - Improve Research Reproducibility

Immunofluorescence staining - Vibratome Sections for Oligodendrocyte morphological analysis

Author: Matthew Swire
Date: Dec 6, 2019，Views：10015

Original protocol used in research article：

Endothelin signalling mediates experience-dependent myelination in the CNS
eLife, Oct 28, 2019; DOI: 10.7554/eLife.49493

General Protocol for Resistance Assays in Liquid Media

Author: Nathaniel J Traaseth
Date: Dec 4, 2019，Views：1099

Original protocol used in research article：

Inducing conformational preference of the membrane protein transporter EmrE through conservative mutations
eLife, Oct 22, 2019; DOI: 10.7554/eLife.48909

C2C12 culture and generation of Gene-modified C2C12 cells

Author: Lin Mei
Date: Dec 4, 2019，Views：1160

Original protocol used in research article：

A mechanism in agrin signaling revealed by a prevalent Rapsyn mutation in congenital myasthenic syndrome
eLife, Sep 24, 2019; DOI: 10.7554/eLife.49180

Quantification of tracheal density in IFMs

Author: Stefan Luschnig
Date: Dec 4, 2019，Views：1158

Original protocol used in research article：

Matrix metalloproteinase 1 modulates invasive behavior of tracheal branches during entry into Drosophila flight muscles
eLife, Oct 2, 2019; DOI: 10.7554/eLife.48857

Growth chamber experiments

Author: Steven Penfield
Date: Dec 4, 2019，Views：1155

Original protocol used in research article：

Flowering time and seed dormancy control use external coincidence to generate life history strategy
eLife, Mar 31, 2015; DOI: 10.7554/eLife.05557

Isolation of skeletal muscle-derived mononuclear cells

Author: So-ichiro Fukada
Date: Dec 3, 2019，Views：1050

Original protocol used in research article：

Sustained expression of HeyL is critical for the proliferation of muscle stem cells in overloaded muscle
eLife, Sep 23, 2019; DOI: 10.7554/eLife.48284

Purification of mammalian dynein-dynactin

Author: Ahmet Yildiz
Date: Dec 3, 2019，Views：1127

Original protocol used in research article：

Kinesin and dynein use distinct mechanisms to bypass obstacles
eLife, Sep 9, 2019; DOI: 10.7554/eLife.48629

In vitro differentiation of mESCs in embryoid bodies

Author: Lars Schomacher
Date: Dec 3, 2019，Views：1190

Original protocol used in research article：

NEIL1 and NEIL2 DNA glycosylases protect neural crest development against mitochondrial oxidative stress
eLife, Sep 30, 2019; DOI: 10.7554/eLife.49044

Platelet isolation and quantification

Author: Mercedes Costell
Date: Dec 1, 2019，Views：1091

Original protocol used in research article：

The fibronectin synergy site re-enforces cell adhesion and mediates a crosstalk between integrin classes
eLife, Jan 16, 2017; DOI: 10.7554/eLife.22264

Preparation of mini-Gs heterotrimer

Author: Christopher Gordon Tate
Date: Dec 1, 2019，Views：1689

Original protocol used in research article：

Cryo-EM structure of the adenosine A_2A receptor coupled to an engineered heterotrimeric G protein
eLife, May 4, 2018; DOI: 10.7554/eLife.35946

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