Bio-101 - Improve Research Reproducibility

In situ hybridization protocol

Author: Peng Huang
Date: Dec 11, 2019，Views：1060

Original protocol used in research article：

Notch signalling maintains Hedgehog responsiveness via a Gli-dependent mechanism during spinal cord patterning in zebrafish
eLife, Aug 27, 2019; DOI: 10.7554/eLife.49252

3D reconstruction (and others)

Author: David M. Miller
Date: Dec 11, 2019，Views：1079

Original protocol used in research article：

C. elegans neurons have functional dendritic spines
eLife, Oct 4, 2019; DOI: 10.7554/eLife.47918

Genotyping

Author: Cunming Duan
Date: Dec 11, 2019，Views：1163

Original protocol used in research article：

Cell-autonomous regulation of epithelial cell quiescence by calcium channel Trpv6
eLife, Sep 17, 2019; DOI: 10.7554/eLife.48003

Analysis of spatial scale sizes

Author: Michael Peer
Date: Dec 11, 2019，Views：1056

Original protocol used in research article：

Processing of different spatial scales in the human brain
eLife, Sep 10, 2019; DOI: 10.7554/eLife.47492

Cuticle preparations

Authors: Yoseop Yoon and Urs Schmidt-Ott
Date: Dec 11, 2019，Views：1333

Original protocol used in research article：

Embryo polarity in moth flies and mosquitoes relies on distinct old genes with localized transcript isoforms
eLife, Oct 8, 2019; DOI: 10.7554/eLife.46711

Generation of HeLa KO cells with CRISPR

Authors: Florian Ullrich and Thomas J Jentsch
Date: Dec 11, 2019，Views：1166

Original protocol used in research article：

Identification of TMEM206 proteins as pore of PAORAC/ASOR acid-sensitive chloride channels
eLife, Jul 18, 2019; DOI: 10.7554/eLife.49187

Imaging Orc1 cellular dynamics in Drosophila S2 cells

Author: Michael R Botchan
Date: Dec 10, 2019，Views：1062

Original protocol used in research article：

A new class of disordered elements controls DNA replication through initiator self-assembly
eLife, Sep 27, 2019; DOI: 10.7554/eLife.48562

Large-scale metabolome analysis

Authors: Pamela Maher and Antonio Currais
Date: Dec 9, 2019，Views：1459

Original protocol used in research article：

Elevating acetyl-CoA levels reduces aspects of brain aging
eLife, Nov 19, 2019; DOI: 10.7554/eLife.47866

Respiration measurements

Author: David C Chan
Date: Dec 8, 2019，Views：1141

Original protocol used in research article：

Mitochondrial fusion is required for spermatogonial differentiation and meiosis
eLife, Oct 9, 2019; DOI: 10.7554/eLife.51601

Drosha cleavage assay

Author: Tsui Han Loo
Date: Dec 6, 2019，Views：1169

Original protocol used in research article：

The mammalian LINC complex component SUN1 regulates muscle regeneration by modulating drosha activity
eLife, Nov 5, 2019; DOI: 10.7554/eLife.49485

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