Bio-101 - Improve Research Reproducibility

Single molecule RNA fluorescent in situ hybridization (FISH) and imaging

Author: Michele L Lemons
Date: Jan 6, 2020，Views：1079

Original protocol used in research article：

Neurexin directs partner-specific synaptic connectivity in C. elegans
eLife, Jul 24, 2018; DOI: 10.7554/eLife.35692

Stability calculations

Author: Amelie Stein
Date: Dec 30, 2019，Views：1147

Original protocol used in research article：

Computational and cellular studies reveal structural destabilization and degradation of MLH1 variants in Lynch syndrome
eLife, Nov 7, 2019; DOI: 10.7554/eLife.49138

FRET measurement

Authors: Wanli Liu, Wei Chen and Jizhong Lou
Date: Dec 29, 2019，Views：1254

Original protocol used in research article：

FcγRIIB-I232T polymorphic change allosterically suppresses ligand binding
eLife, Jul 25, 2019; DOI: 10.7554/eLife.46689

Laser speckle perfusion measurements of brain

Author: Stefan Offermanns
Date: Dec 29, 2019，Views：2289

Original protocol used in research article：

Myogenic vasoconstriction requires G₁₂/G₁₃ and LARG to maintain local and systemic vascular resistance
eLife, Sep 24, 2019; DOI: 10.7554/eLife.49374

5’/3’ RACE

Author: Judith Wexler
Date: Dec 24, 2019，Views：1305

Original protocol used in research article：

Hemimetabolous insects elucidate the origin of sexual development via alternative splicing
eLife, Sep 3, 2019; DOI: 10.7554/eLife.47490

β-galactosidase detection in embryos and tissues using X-Gal

Author: Shahragim Tajbakhsh
Date: Dec 22, 2019，Views：993

Original protocol used in research article：

A distinct cardiopharyngeal mesoderm genetic hierarchy establishes antero-posterior patterning of esophagus striated muscle
eLife, Sep 19, 2019; DOI: 10.7554/eLife.47460

Electrophoretic mobility shift assay

Author: Yan Zhang
Date: Dec 19, 2019，Views：1097

Original protocol used in research article：

Tyr1 phosphorylation promotes phosphorylation of Ser2 on the C-terminal domain of eukaryotic RNA polymerase II by P-TEFb
eLife, Aug 6, 2019; DOI: 10.7554/eLife.48725

Single cell isolation and flow cytometry for scRNA-Seq from utricular sensory epithelia

Authors: Mirko Scheibinger, Stefan Heller and Peter G Barr-Gillespie
Date: Dec 17, 2019，Views：1155

Original protocol used in research article：

Single-cell proteomics reveals changes in expression during hair-cell development
eLife, Nov 4, 2019; DOI: 10.7554/eLife.50777

Intact protein mass spectrometry

Author: Michael A Marletta
Date: Dec 17, 2019，Views：1741

Original protocol used in research article：

Allosteric activation of the nitric oxide receptor soluble guanylate cyclase mapped by cryo-electron microscopy
eLife, Sep 30, 2019; DOI: 10.7554/eLife.50634

Natural transformation assay

Author: Melanie Blokesch
Date: Dec 17, 2019，Views：1230

Original protocol used in research article：

Neighbor predation linked to natural competence fosters the transfer of large genomic regions in Vibrio cholerae
eLife, Sep 3, 2019; DOI: 10.7554/eLife.48212

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