Bio-101 - Improve Research Reproducibility

Fluorometry of solubilized cell extracts

Author: Seksiri Arttamangkul
Date: Dec 16, 2019，Views：998

Original protocol used in research article：

Visualizing endogenous opioid receptors in living neurons using ligand-directed chemistry
eLife, Oct 7, 2019; DOI: 10.7554/eLife.49319

Live-cell imaging

Author: Elçin Ünal
Date: Dec 16, 2019，Views：1097

Original protocol used in research article：

Meiotic cellular rejuvenation is coupled to nuclear remodeling in budding yeast
eLife, Aug 9, 2019; DOI: 10.7554/eLife.47156

Quantification of neural crest contributions to the ventricular myocardium and regeneration

Author: Marianne E Bronner
Date: Dec 14, 2019，Views：1291

Original protocol used in research article：

Cardiac neural crest contributes to cardiomyocytes in amniotes and heart regeneration in zebrafish
eLife, Aug 8, 2019; DOI: 10.7554/eLife.47929

Flow cytometry blood staining protocol for BLT humanized mice

Author: Collin Kieffer
Date: Dec 13, 2019，Views：1189

Original protocol used in research article：

Mechanisms of virus dissemination in bone marrow of HIV-1–infected humanized BLT mice
eLife, Oct 28, 2019; DOI: 10.7554/eLife.46916

Droplet capture of single nuclei, cDNA sequencing and data analysis

Author: Nicholas Ryba
Date: Dec 13, 2019，Views：1559

Original protocol used in research article：

Stereotyped transcriptomic transformation of somatosensory neurons in response to injury
eLife, Oct 8, 2019; DOI: 10.7554/eLife.49679

Generation of transgenic fish and chemical treatment

Author: Bo Zhang
Date: Dec 13, 2019，Views：1068

Original protocol used in research article：

One-step efficient generation of dual-function conditional knockout and geno-tagging alleles in zebrafish
eLife, Oct 30, 2019; DOI: 10.7554/eLife.48081

Metabolite extraction and LC-MS analysis

Author: Eugene I Shakhnovich
Date: Dec 12, 2019，Views：1084

Original protocol used in research article：

Adaptation to mutational inactivation of an essential gene converges to an accessible suboptimal fitness peak
eLife, Oct 1, 2019; DOI: 10.7554/eLife.50509

ChIP-Sequencing

Author: Neal A Deluca
Date: Dec 12, 2019，Views：1481

Original protocol used in research article：

Herpes simplex viral nucleoprotein creates a competitive transcriptional environment facilitating robust viral transcription and host shut off
eLife, Oct 22, 2019; DOI: 10.7554/eLife.51109

Four-quadrant behavioral assay

Authors: Christopher J Potter and Chun-Chieh Lin
Date: Dec 12, 2019，Views：1167

Original protocol used in research article：

Food odors trigger Drosophila males to deposit a pheromone that guides aggregation and female oviposition decisions
eLife, Sep 30, 2015; DOI: 10.7554/eLife.08688

In silico analyses of SecA pore dynamics

Author: Ian Collinson
Date: Dec 12, 2019，Views：1097

Original protocol used in research article：

HDX-MS reveals nucleotide-dependent, anti-correlated opening and closure of SecA and SecY channels of the bacterial translocon
eLife, Jul 10, 2019; DOI: 10.7554/eLife.47402

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