Bio-101 - Improve Research Reproducibility

Assembly of stalled yeast Pol II transcription elongation complex

Authors: Zhijie Chen, Ronen Gabizon, Tingting Yao and Carlos Bustamante
Date: Feb 23, 2020，Views：1381

Original protocol used in research article：

High-resolution and high-accuracy topographic and transcriptional maps of the nucleosome barrier
eLife, Jul 31, 2019; DOI: 10.7554/eLife.48281

Subcellular fractionation

Author: Rosa Puertollano
Date: Feb 21, 2020，Views：1286

Original protocol used in research article：

The transcription factors TFE3 and TFEB amplify p53 dependent transcriptional programs in response to DNA damage
eLife, Dec 6, 2018; DOI: 10.7554/eLife.40856

Primary NSC Culture

Authors: Isabelle Maria Blomfield and François Guillemot
Date: Feb 21, 2020，Views：1310

Original protocol used in research article：

Id4 promotes the elimination of the pro-activation factor Ascl1 to maintain quiescence of adult hippocampal stem cells
eLife, Sep 25, 2019; DOI: 10.7554/eLife.48561

Juxtacellular labelling of single neurons

Author: Navid Farassat
Date: Feb 21, 2020，Views：1161

Original protocol used in research article：

In vivo functional diversity of midbrain dopamine neurons within identified axonal projections
eLife, Oct 3, 2019; DOI: 10.7554/eLife.48408

Chromatin immunoprecipitation (ChIP)

Authors: Xuehua Zhong and Xiangsong Chen
Date: Feb 20, 2020，Views：1299

Original protocol used in research article：

POWERDRESS interacts with HISTONE DEACETYLASE 9 to promote aging in Arabidopsis
eLife, Nov 22, 2016; DOI: 10.7554/eLife.17214

Automatic determination of optimal cluster number

Author: Jakob Nikolas Kather
Date: Feb 20, 2020，Views：1201

Original protocol used in research article：

Topography of cancer-associated immune cells in human solid tumors
eLife, Sep 4, 2018; DOI: 10.7554/eLife.36967

α-syn purification

Author: Smriti Sangwan
Date: Feb 20, 2020，Views：1145

Original protocol used in research article：

Inhibition of synucleinopathic seeding by rationally designed inhibitors
eLife, Jan 2, 2020; DOI: 10.7554/eLife.46775

mRNA preparation

Author: Maaike Welling
Date: Feb 20, 2020，Views：3561

Original protocol used in research article：

Primed Track, high-fidelity lineage tracing in mouse pre-implantation embryos using primed conversion of photoconvertible proteins
eLife, Jan 21, 2019; DOI: 10.7554/eLife.44491

In vitro kinase assay using immunoprecipitated Cdk5 from SCN

Authors: Andrea Brenna and Urs Albrecht
Date: Feb 20, 2020，Views：1221

Original protocol used in research article：

Cyclin-dependent kinase 5 (CDK5) regulates the circadian clock
eLife, Nov 5, 2019; DOI: 10.7554/eLife.50925

Live imaging of tracheal invasion into pupal IFMs

Authors: Stefan Luschnig and Julia Sauerwald
Date: Feb 19, 2020，Views：1148

Original protocol used in research article：

Matrix metalloproteinase 1 modulates invasive behavior of tracheal branches during entry into Drosophila flight muscles
eLife, Oct 2, 2019; DOI: 10.7554/eLife.48857

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