Protocols shared by authors via RaP service
Journal
Yeast strain construction and transformation
Authors: Deze Kong and Christina D. Smolke
Date: Feb 7, 2021，Views：964
Original protocol used in research article：
Discovery of a previously unknown biosynthetic capacity of naringenin chalcone synthase by heterologous expression of a tomato gene cluster in yeast
Science Advances, Oct 29, 2020; DOI: 10.1126/sciadv.abd1143
Cell death, membrane depolarization, and membrane fluidity quantification assays
Author: Julia Bos
Date: Feb 5, 2021，Views：823
Original protocol used in research article：
Real-time tracking of bacterial membrane vesicles reveals enhanced membrane traffic upon antibiotic exposure
Science Advances, Jan 20, 2021; DOI: 10.1126/sciadv.abd1033
CRISPR/Cas9 Flag knockin
Authors: Joana G Marques and Beat W Schäfer
Date: Feb 5, 2021，Views：1048
Original protocol used in research article：
NuRD subunit CHD4 regulates super-enhancer accessibility in rhabdomyosarcoma and represents a general tumor dependency
eLife, Aug 3, 2020; DOI: 10.7554/eLife.54993
AAV vectors and stereotactic microinjections
Author: Esther Visser
Date: Feb 5, 2021，Views：780
Original protocol used in research article：
A persistent alcohol cue memory trace drives relapse to alcohol seeking after prolonged abstinence
Science Advances, May 5, 2020; DOI: 10.1126/sciadv.aax7060
Co-expression analysis
Authors: Shuqing Xu, Ian T. Baldwin and Jiancai Li
Date: Feb 4, 2021，Views：959
Original protocol used in research article：
Controlled hydroxylations of diterpenoids allow for plant chemical defense without autotoxicity
Science, Jan 15, 2021; DOI: 10.1126/science.abe4713
Fluctuation assays
Author: R Blake Billmyre
Date: Feb 4, 2021，Views：920
Original protocol used in research article：
Natural mismatch repair mutations mediate phenotypic diversity and drug resistance in Cryptococcus deuterogattii
eLife, Sep 26, 2017; DOI: 10.7554/eLife.28802
Detailed reprogramming protocol
Author: Avani Yeola
Date: Feb 4, 2021，Views：862
Original protocol used in research article：
Induction of muscle-regenerative multipotent stem cells from human adipocytes by PDGF-AB and 5-azacytidine
Science Advances, Jan 13, 2021; DOI: 10.1126/sciadv.abd1929
Animal Study
Author: Thomas F. Rogers
Date: Feb 4, 2021，Views：885
Original protocol used in research article：
Isolation of potent SARS-CoV-2 neutralizing antibodies and protection from disease in a small animal model
Science, Aug 21, 2020; DOI: 10.1126/science.abc7520
Exploratory bifactor analysis
Authors: Christopher Gagne and Sonia J Bishop
Date: Feb 4, 2021，Views：955
Original protocol used in research article：
Impaired adaptation of learning to contingency volatility in internalizing psychopathology
eLife, Dec 22, 2020; DOI: 10.7554/eLife.61387
SUMOylation and in vitro SUMOylation assay
Author: Xiancai Ma
Date: Feb 4, 2021，Views：847
Original protocol used in research article：
TRIM28 promotes HIV-1 latency by SUMOylating CDK9 and inhibiting P-TEFb
eLife, Jan 17, 2019; DOI: 10.7554/eLife.42426
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