Bio-101 - Improve Research Reproducibility

CRISPR constructs

Author: Kui Li
Date: Jan 12, 2021，Views：894

Original protocol used in research article：

CD163 and pAPN double-knockout pigs are resistant to PRRSV and TGEV and exhibit decreased susceptibility to PDCoV while maintaining normal production performance
eLife, Sep 2, 2020; DOI: 10.7554/eLife.57132

Immunofluorescence

Author: Shanshan Gao
Date: Jan 12, 2021，Views：763

Original protocol used in research article：

TIP60 K430 SUMOylation attenuates its interaction with DNA-PKcs in S-phase cells: Facilitating homologous recombination and emerging target for cancer therapy
Science Advances, Jul 9, 2020; DOI: 10.1126/sciadv.aba7822

Study design

Author: Hubert H. Lim
Date: Jan 11, 2021，Views：874

Original protocol used in research article：

Bimodal neuromodulation combining sound and tongue stimulation reduces tinnitus symptoms in a large randomized clinical study
Science Translational Medicine, Oct 6, 2020; DOI: 10.1126/scitranslmed.abb2830

Search for PIN family members from different plant species

Authors: Yuzhou Zhang and Lanxin Li
Date: Jan 11, 2021，Views：828

Original protocol used in research article：

Functional innovations of PIN auxin transporters mark crucial evolutionary transitions during rise of flowering plants
Science Advances, Dec 11, 2020; DOI: 10.1126/sciadv.abc8895

Retrograde DiI labeling

Authors: William Olson and Wenqin Luo
Date: Jan 11, 2021，Views：1539

Original protocol used in research article：

Sparse genetic tracing reveals regionally specific functional organization of mammalian nociceptors
eLife, Oct 12, 2017; DOI: 10.7554/eLife.29507

MSA-2 formulation for in vivo dosing

Author: Samanthi A. Perera
Date: Jan 11, 2021，Views：918

Original protocol used in research article：

An orally available non-nucleotide STING agonist with antitumor activity
Science, Aug 20, 2020; DOI: 10.1126/science.aba6098

Culture and treatment of BMDMs

Author: Wang Yi
Date: Jan 11, 2021，Views：990

Original protocol used in research article：

MBD2 serves as a viable target against pulmonary fibrosis by inhibiting macrophage M2 program
Science Advances, Jan 1, 2021; DOI: 10.1126/sciadv.abb6075

Purification of human mitoribosome from HEK cell

Authors: Hanting Yang and Nirupa Desai
Date: Jan 10, 2021，Views：1099

Original protocol used in research article：

Elongational stalling activates mitoribosome-associated quality control
Science, Nov 26, 2020; DOI: 10.1126/science.abc7782

In vitro Anti-tumor TIL expansion experiment

Author: Sri Krishna
Date: Jan 8, 2021，Views：1007

Original protocol used in research article：

Stem-like CD8 T cells mediate response of adoptive cell immunotherapy against human cancer
Science, Dec 11, 2020; DOI: 10.1126/science.abb9847

Illumina sequencing and SNP calling

Author: Qingyun Liu
Date: Jan 7, 2021，Views：799

Original protocol used in research article：

Mycobacterium tuberculosis clinical isolates carry mutational signatures of host immune environments
Science Advances, May 28, 2020; DOI: 10.1126/sciadv.aba4901

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