Bio-101 - Improve Research Reproducibility

HCR fluorescent in situ hybridization (FISH) on slides

Author: Natasha M O'Brown
Date: Jan 29, 2021，Views：1395

Original protocol used in research article：

Suppression of transcytosis regulates zebrafish blood-brain barrier function
eLife, Aug 20, 2019; DOI: 10.7554/eLife.47326

Whole-mount tail skin staining and imaging

Authors: Zijian Xu and Ting Chen
Date: Jan 29, 2021，Views：1191

Original protocol used in research article：

Embryonic attenuated Wnt/β-catenin signaling defines niche location and long-term stem cell fate in hair follicle
eLife, Dec 14, 2015; DOI: 10.7554/eLife.10567

Image analysis

Author: Ludovico Cantuti-Castelvetri
Date: Jan 28, 2021，Views：901

Original protocol used in research article：

Neuropilin-1 facilitates SARS-CoV-2 cell entry and infectivity
Science, Nov 13, 2020; DOI: 10.1126/science.abd2985

Matrigel Invasion Assay Protocol

Authors: Chen Dong, Pooja Popli, Ramakrishna Kommagani and Thorold W Theunissen
Date: Jan 28, 2021，Views：7912

Original protocol used in research article：

Derivation of trophoblast stem cells from naïve human pluripotent stem cells
eLife, Feb 12, 2020; DOI: 10.7554/eLife.52504

X-ray crystallographic analysis of the WT erα LBD-BZA complex

Author: Sean W Fanning
Date: Jan 28, 2021，Views：800

Original protocol used in research article：

The SERM/SERD bazedoxifene disrupts ESR1 helix 12 to overcome acquired hormone resistance in breast cancer cells
eLife, Nov 29, 2018; DOI: 10.7554/eLife.37161

Synthesis and functionalization of AgNPs

Author: Ludovico Cantuti-Castelvetri
Date: Jan 28, 2021，Views：945

Original protocol used in research article：

Neuropilin-1 facilitates SARS-CoV-2 cell entry and infectivity
Science, Nov 13, 2020; DOI: 10.1126/science.abd2985

In vitro pull down and Co-IP assays

Authors: Claudia Castillo-González and Xiuren Zhang
Date: Jan 28, 2021，Views：2573

Original protocol used in research article：

Geminivirus-encoded TrAP suppressor inhibits the histone methyltransferase SUVH4/KYP to counter host defense
eLife, Sep 7, 2015; DOI: 10.7554/eLife.06671

Animals, human tissue, and mouse cells

Author: Khaled S. Abd-Elrahman
Date: Jan 28, 2021，Views：776

Original protocol used in research article：

A\β oligomers induce pathophysiological mGluR5 signaling in Alzheimer\’s disease model mice in a sex-selective manner
Science Signaling, Dec 14, 2020; DOI: 10.1126/scisignal.abd2494

Western blot assay

Authors: Leiming Wang, Sophia Y. Tsai and Ming-Jer Tsai
Date: Jan 28, 2021，Views：966

Original protocol used in research article：

Small-molecule inhibitor targeting orphan nuclear receptor COUP-TFII for prostate cancer treatment
Science Advances, Apr 28, 2020; DOI: 10.1126/sciadv.aaz8031

Electrophysiology

Author: Wallace B Thoreson
Date: Jan 27, 2021，Views：837

Original protocol used in research article：

Ca²⁺ sensor synaptotagmin-1 mediates exocytosis in mammalian photoreceptors
eLife, Jun 7, 2019; DOI: 10.7554/eLife.45946

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