Bio-101 - Improve Research Reproducibility

Assessment of thrombolysis efficacy in a mouse model

Authors: Yu Gao and Wang si yu
Date: Feb 3, 2021，Views：836

Original protocol used in research article：

Accelerating thrombolysis using a precision and clot-penetrating drug delivery strategy by nanoparticle-shelled microbubbles
Science Advances, Jul 29, 2020; DOI: 10.1126/sciadv.aaz8204

Immunohistochemistry protocol for analyzing Hedgehog pathway factors in mouse embryos

Authors: Saishu Yoshida and Kiyotsugu Yoshida
Date: Feb 3, 2021，Views：881

Original protocol used in research article：

The novel ciliogenesis regulator DYRK2 governs Hedgehog signaling during mouse embryogenesis
eLife, Aug 6, 2020; DOI: 10.7554/eLife.57381

tRNA charging assay

Authors: Natalya N Pavlova and Craig B Thompson
Date: Feb 3, 2021，Views：1654

Original protocol used in research article：

Translation in amino-acid-poor environments is limited by tRNA^Gln charging
eLife, Dec 8, 2020; DOI: 10.7554/eLife.62307

Global protein synthesis quantification in Caenorhabditis elegans by puromycin incorporation

Authors: Clemens Heissenberger and Markus Schosserer
Date: Feb 3, 2021，Views：1082

Original protocol used in research article：

The ribosomal RNA m⁵C methyltransferase NSUN-1 modulates healthspan and oogenesis in Caenorhabditis elegans
eLife, Dec 8, 2020; DOI: 10.7554/eLife.56205

Sperm motility

Authors: Sylvia Fechner, U Benjamin Kaupp and Reinhard Seifert
Date: Feb 3, 2021，Views：851

Original protocol used in research article：

A K⁺-selective CNG channel orchestrates Ca²⁺ signalling in zebrafish sperm
eLife, Dec 9, 2015; DOI: 10.7554/eLife.07624

Animal immunization and challenge studies

Authors: Shibo Jiang, Shihui Sun and Cheng-Feng Qin
Date: Feb 3, 2021，Views：941

Original protocol used in research article：

Adaptation of SARS-CoV-2 in BALB/c mice for testing vaccine efficacy
Science, Sep 24, 2020; DOI: 10.1126/science.abc4730

Construction, expression, and purification of recombinant RBD-Fc.

Authors: cheng-feng Qin, shihui sun and shibo jiang
Date: Feb 3, 2021，Views：884

Original protocol used in research article：

Adaptation of SARS-CoV-2 in BALB/c mice for testing vaccine efficacy
Science, Sep 24, 2020; DOI: 10.1126/science.abc4730

Lifespan assay

Authors: Jongsun Lee, Sieun S. and Seung-Jae V.
Date: Feb 2, 2021，Views：781

Original protocol used in research article：

A mutation that alters the 255th glutamate to lysine in RSKS-1/S6 kinase reliably extends the lifespan of C. elegans
microPublication Biology, Oct 27, 2020; DOI: 10.17912/micropub.biology.000322

Dissection

Authors: Vera D Rinaldi and Oliver J Rando
Date: Feb 2, 2021，Views：872

Original protocol used in research article：

An atlas of cell types in the mouse epididymis and vas deferens
eLife, Jul 30, 2020; DOI: 10.7554/eLife.55474

Gene set enrichment analysis (GSEA) of sex-biased genes

Authors: Meritxell Oliva, Barbara E. Stranger and Daniel Cotter
Date: Feb 2, 2021，Views：979

Original protocol used in research article：

The impact of sex on gene expression across human tissues
Science, Sep 11, 2020; DOI: 10.1126/science.aba3066

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