Protocols shared by authors via RaP service
Journal
DNA library preparation
Authors: Ryan Muller and Nicholas T. Ingolia
Date: Jan 24, 2021，Views：1048
Original protocol used in research article：
CiBER-seq dissects genetic networks by quantitative CRISPRi profiling of expression phenotypes
Science, Dec 11, 2020; DOI: 10.1126/science.abb9662
Plasmid library construction
Authors: Ryan Muller and Nicholas T Ingolia
Date: Jan 24, 2021，Views：1080
Original protocol used in research article：
CiBER-seq dissects genetic networks by quantitative CRISPRi profiling of expression phenotypes
Science, Dec 11, 2020; DOI: 10.1126/science.abb9662
Genomic annotation files preparation
Author: Mohamed Nadhir Djekidel
Date: Jan 24, 2021，Views：1363
Original protocol used in research article：
A transcriptional roadmap for 2C-like–to–pluripotent state transition
Science Advances, May 28, 2020; DOI: 10.1126/sciadv.aay5181
Extracorporeal circulation treatment
Author: He-Xin Yan
Date: Jan 24, 2021，Views：742
Original protocol used in research article：
An extracorporeal bioartificial liver embedded with 3D-layered human liver progenitor-like cells relieves acute liver failure in pigs
Science Translational Medicine, Jul 7, 2020; DOI: 10.1126/scitranslmed.aba5146
Cell culture and hepatocytes optimization
Author: Weijian Li
Date: Jan 24, 2021，Views：787
Original protocol used in research article：
An extracorporeal bioartificial liver embedded with 3D-layered human liver progenitor-like cells relieves acute liver failure in pigs
Science Translational Medicine, Jul 7, 2020; DOI: 10.1126/scitranslmed.aba5146
Repeat pseudo-genome preparation
Author: Mohamed Nadhir Djekidel
Date: Jan 23, 2021，Views：1069
Original protocol used in research article：
A transcriptional roadmap for 2C-like–to–pluripotent state transition
Science Advances, May 28, 2020; DOI: 10.1126/sciadv.aay5181
Immunohistochemistry and imaging
Author: Mehak M Khan
Date: Jan 23, 2021，Views：815
Original protocol used in research article：
Loss of Doc2b does not influence transmission at Purkinje cell to deep nuclei synapses under physiological conditions
eLife, Apr 29, 2020; DOI: 10.7554/eLife.55165
Expression variability estimation
Author: Benjamin Jung Fair
Date: Jan 22, 2021，Views：946
Original protocol used in research article：
Gene expression variability in human and chimpanzee populations share common determinants
eLife, Oct 21, 2020; DOI: 10.7554/eLife.59929
In vitro cellular experiments
Author: Jingjing Liang
Date: Jan 19, 2021，Views：856
Original protocol used in research article：
Nanoparticle-enhanced chemo-immunotherapy to trigger robust antitumor immunity
Science Advances, Aug 27, 2020; DOI: 10.1126/sciadv.abc3646
Microgel fabrication and tethering of SA-PD-L1
Authors: María M. Coronel, Haval Shirwan and Andrés J. García
Date: Jan 19, 2021，Views：920
Original protocol used in research article：
Immunotherapy via PD-L1–presenting biomaterials leads to long-term islet graft survival
Science Advances, Aug 27, 2020; DOI: 10.1126/sciadv.aba5573
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