Bio-101 - Improve Research Reproducibility

PG9 chimeric light chain IgG expression

Author: Roberta P Fuller
Date: Jan 7, 2021，Views：966

Original protocol used in research article：

Reprogramming the antigen specificity of B cells using genome-editing technologies
eLife, Jan 17, 2019; DOI: 10.7554/eLife.42995

Measurement of CD8+ T cell cytotoxicity

Authors: Meixi Hao, Caoyun Ju and Can Zhang
Date: Jan 6, 2021，Views：834

Original protocol used in research article：

Combination of metabolic intervention and T cell therapy enhances solid tumor immunotherapy
Science Translational Medicine, Nov 24, 2020; DOI: 10.1126/scitranslmed.aaz6667

Haemocytometry

Authors: Joachim Linssen and Andre J van der Ven
Date: Jan 5, 2021，Views：1051

Original protocol used in research article：

A novel haemocytometric COVID-19 prognostic score developed and validated in an observational multicentre European hospital-based study
eLife, Nov 26, 2020; DOI: 10.7554/eLife.63195

Generation of FAM150A-conditioned and FAM150B-conditioned media

Author: Jikui Guan
Date: Jan 5, 2021，Views：879

Original protocol used in research article：

FAM150A and FAM150B are activating ligands for anaplastic lymphoma kinase
eLife, Sep 29, 2015; DOI: 10.7554/eLife.09811

Shelf life testing of antibody-functionalized microchips

Author: Hadi Shafiee
Date: Jan 4, 2021，Views：888

Original protocol used in research article：

Virus detection using nanoparticles and deep neural network–enabled smartphone system
Science Advances, Dec 16, 2020; DOI: 10.1126/sciadv.abd5354

In silico SETD2 methylation site analysis

Authors: Riyad N. H. Seervai and Cheryl Lyn Walker
Date: Jan 1, 2021，Views：820

Original protocol used in research article：

The Huntingtin-interacting protein SETD2/HYPB is an actin lysine methyltransferase
Science Advances, Oct 1, 2020; DOI: 10.1126/sciadv.abb7854

Electromagnetic simulation

Authors: Jahyun Koo and Xie Zhaoqian
Date: Jan 1, 2021，Views：747

Original protocol used in research article：

Wirelessly controlled bioresorbable drug delivery device with active valves that exploit electrochemically triggered crevice corrosion
Science Advances, Aug 27, 2020; DOI: 10.1126/sciadv.abb1093

Differential analysis of N-glycopeptide abundance and N-glycosylation site occupancy for studying protein N-glycosylation dysregulation in human disease

Authors: Qi Zhang, Cheng Ma, Lian Li and Lih-Shen Chin
Date: Dec 30, 2020，Views：1048

Original protocol used in research article：

Integrative glycoproteomics reveals protein N-glycosylation aberrations and glycoproteomic network alterations in Alzheimer’s disease
Science Advances, Oct 1, 2020; DOI: 10.1126/sciadv.abc5802

RNA-seq expression analysis

Authors: Stefan Bohn and Nevan J. Krogan
Date: Dec 30, 2020，Views：1279

Original protocol used in research article：

Genetic interaction mapping informs integrative structure determination of protein complexes
Science, Dec 11, 2020; DOI: 10.1126/science.aaz4910

Generation of TILs

Author: Sri Krishna
Date: Dec 29, 2020，Views：995

Original protocol used in research article：

Stem-like CD8 T cells mediate response of adoptive cell immunotherapy against human cancer
Science, Dec 11, 2020; DOI: 10.1126/science.abb9847

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