Bio-101 - Improve Research Reproducibility

Y-maze, modified and spontaneous alternation task version

Authors: Da Hee Jung and Byung Tae Choi
Date: Nov 23, 2020，Views：5043

Original protocol used in research article：

Therapeutic effects of anodal transcranial direct current stimulation in a rat model of ADHD
eLife, Sep 21, 2020; DOI: 10.7554/eLife.56359

WGCNA network analysis

Author: Kathleen Greenham
Date: Nov 23, 2020，Views：1129

Original protocol used in research article：

Temporal network analysis identifies early physiological and transcriptomic indicators of mild drought in Brassica rapa
eLife, Aug 18, 2017; DOI: 10.7554/eLife.29655

‘On-bead’ peptidisc reconstitution

Author: Franck Duong
Date: Nov 23, 2020，Views：1000

Original protocol used in research article：

The Peptidisc, a simple method for stabilizing membrane proteins in detergent-free solution
eLife, Aug 15, 2018; DOI: 10.7554/eLife.34085

Western blot analysis of cultured fibroblasts

Author: Philip S. Low
Date: Nov 22, 2020，Views：797

Original protocol used in research article：

Targeted inhibition of PI3 kinase/mTOR specifically in fibrotic lung fibroblasts suppresses pulmonary fibrosis in experimental models
Science Translational Medicine, Oct 27, 2020; DOI: 10.1126/scitranslmed.aay3724

Recombinant IRF5 dimerization assay - protein preparation

Author: Gang Chen
Date: Nov 20, 2020，Views：1108

Original protocol used in research article：

Inhibition of IRF5 cellular activity with cell-penetrating peptides that target homodimerization
Science Advances, May 14, 2020; DOI: 10.1126/sciadv.aay1057

Calculating scores and classifications

Author: Melissa Chiasson
Date: Nov 20, 2020，Views：826

Original protocol used in research article：

Multiplexed measurement of variant abundance and activity reveals VKOR topology, active site and human variant impact
eLife, Sep 1, 2020; DOI: 10.7554/eLife.58026

Specificity scores

Authors: Silvia Domcke and Andrew J. Hill
Date: Nov 20, 2020，Views：921

Original protocol used in research article：

A human cell atlas of fetal chromatin accessibility
Science, Nov 13, 2020; DOI: 10.1126/science.aba7612

SARS-CoV-2 neutralization assay

Author: Jesse H. Erasmus
Date: Nov 20, 2020，Views：933

Original protocol used in research article：

An Alphavirus-derived replicon RNA vaccine induces SARS-CoV-2 neutralizing antibody and T cell responses in mice and nonhuman primates
Science Translational Medicine, Aug 4, 2020; DOI: 10.1126/scitranslmed.abc9396

LION formulation of the repRNA vaccine

Author: Jesse H. Erasmus
Date: Nov 20, 2020，Views：918

Original protocol used in research article：

An Alphavirus-derived replicon RNA vaccine induces SARS-CoV-2 neutralizing antibody and T cell responses in mice and nonhuman primates
Science Translational Medicine, Aug 4, 2020; DOI: 10.1126/scitranslmed.abc9396

Computational prediction of RNA structures

Author: Yan Zhang
Date: Nov 19, 2020，Views：938

Original protocol used in research article：

Operon mRNAs are organized into ORF-centric structures that predict translation efficiency
eLife, Jan 31, 2017; DOI: 10.7554/eLife.22037

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