Bio-101 - Improve Research Reproducibility

Motor unit characteristics

Authors: A. Del Vecchio and D. Farina
Date: Dec 2, 2020，Views：768

Original protocol used in research article：

Spinal motoneurons of the human newborn are highly synchronized during leg movements
Science Advances, Nov 19, 2020; DOI: 10.1126/sciadv.abc3916

Preparation of single hemocytes in suspension

Authors: Sudhir Gopal Tattikota and Norbert Perrimon
Date: Dec 2, 2020，Views：1172

Original protocol used in research article：

A single-cell survey of Drosophila blood
eLife, May 12, 2020; DOI: 10.7554/eLife.54818

APEX2 reaction

Authors: Luis Bonet-Ponce and Mark R. Cookson
Date: Dec 2, 2020，Views：880

Original protocol used in research article：

LRRK2 mediates tubulation and vesicle sorting from lysosomes
Science Advances, Nov 11, 2020; DOI: 10.1126/sciadv.abb2454

APAP-ALF patient plasma analysis

Authors: Katelyn Arnold and Jian Liu
Date: Dec 2, 2020，Views：874

Original protocol used in research article：

Design of anti-inflammatory heparan sulfate to protect against acetaminophen-induced acute liver failure
Science Translational Medicine, Mar 17, 2020; DOI: 10.1126/scitranslmed.aav8075

Whole-Mount in situ hybridization

Author: Andrea Elizabeth Wills
Date: Dec 1, 2020，Views：858

Original protocol used in research article：

Chromatin accessibility dynamics and single cell RNA-Seq reveal new regulators of regeneration in neural progenitors
eLife, Apr 27, 2020; DOI: 10.7554/eLife.52648

Primers information for quantitative PCR

Authors: Xiao Ma and Xiaolei Xu
Date: Dec 1, 2020，Views：1009

Original protocol used in research article：

Retinoid X receptor alpha is a spatiotemporally predominant therapeutic target for anthracycline-induced cardiotoxicity
Science Advances, Jan 28, 2020; DOI: 10.1126/sciadv.aay2939

Fecal microbiota 16S rRNA gene sequencing

Author: Jakob Stokholm
Date: Dec 1, 2020，Views：812

Original protocol used in research article：

Delivery mode and gut microbial changes correlate with an increased risk of childhood asthma
Science Translational Medicine, Nov 10, 2020; DOI: 10.1126/scitranslmed.aax9929

Marble burying test

Author: Huda Y Zoghbi
Date: Nov 30, 2020，Views：1033

Original protocol used in research article：

Restoration of Mecp2 expression in GABAergic neurons is sufficient to rescue multiple disease features in a mouse model of Rett syndrome
eLife, Jun 21, 2016; DOI: 10.7554/eLife.14198

NMR spectroscopy and structure calculations

Author: Edward K. Gilding
Date: Nov 30, 2020，Views：826

Original protocol used in research article：

Neurotoxic peptides from the venom of the giant Australian stinging tree
Science Advances, Sep 16, 2020; DOI: 10.1126/sciadv.abb8828

Analysis of Ca2+ transients

Author: Katsutoshi Mizuno
Date: Nov 30, 2020，Views：875

Original protocol used in research article：

Role of Ca2+ transients at the node of the mouse embryo in breaking of left-right symmetry
Science Advances, Jul 21, 2020; DOI: 10.1126/sciadv.aba1195

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