Bio-101 - Improve Research Reproducibility

Tracking subcellular localization of surface proteins in Staphylococcus aureus by immunofluorescence microscopy

Authors: Salvatore J. Scaffidi, Mac A. Shebes and Wenqi Yu
Date: Dec 21, 2020，Views：1139

Original protocol used in research article：

Septal secretion of protein A in Staphylococcus aureus requires SecA and lipoteichoic acid synthesis
eLife, May 14, 2018; DOI: 10.7554/eLife.34092

Cell culture and adipocyte differentiation

Authors: Marcelo A Mori and Andréa Livia Rocha
Date: Dec 21, 2020，Views：1698

Original protocol used in research article：

Enoxacin induces oxidative metabolism and mitigates obesity by regulating adipose tissue miRNA expression
Science Advances, Dec 2, 2020; DOI: 10.1126/sciadv.abc6250

Animals and treatments

Author: Hui Xie
Date: Dec 20, 2020，Views：903

Original protocol used in research article：

Ångstrom-scale silver particle–embedded carbomer gel promotes wound healing by inhibiting bacterial colonization and inflammation
Science Advances, Oct 23, 2020; DOI: 10.1126/sciadv.aba0942

Urea avidity ELISA

Author: Harry N White
Date: Dec 19, 2020，Views：1074

Original protocol used in research article：

Variant proteins stimulate more IgM+ GC B-cells revealing a mechanism of cross-reactive recognition by antibody memory
eLife, May 1, 2018; DOI: 10.7554/eLife.26832

Ubiquitylation assays

Author: Joseph S Harrison
Date: Dec 18, 2020，Views：867

Original protocol used in research article：

Hemi-methylated DNA regulates DNA methylation inheritance through allosteric activation of H3 ubiquitylation by UHRF1
eLife, Sep 6, 2016; DOI: 10.7554/eLife.17101

RNA library preparation

Author: Ryan Muller
Date: Dec 16, 2020，Views：971

Original protocol used in research article：

CiBER-seq dissects genetic networks by quantitative CRISPRi profiling of expression phenotypes
Science, Dec 11, 2020; DOI: 10.1126/science.abb9662

Detergent-resistant membrane isolation and cholesterol measurement

Authors: Ayaka Ito, Xin Rong and Peter Tontonoz
Date: Dec 16, 2020，Views：843

Original protocol used in research article：

LXRs link metabolism to inflammation through Abca1-dependent regulation of membrane composition and TLR signaling
eLife, Jul 14, 2015; DOI: 10.7554/eLife.08009

scRNA-seq analysis

Author: Mikael Simons
Date: Dec 16, 2020，Views：954

Original protocol used in research article：

Neuropilin-1 facilitates SARS-CoV-2 cell entry and infectivity
Science, Nov 13, 2020; DOI: 10.1126/science.abd2985

ChIP sequencing and analysis

Author: Francisca Martinez Real
Date: Dec 16, 2020，Views：889

Original protocol used in research article：

The mole genome reveals regulatory rearrangements associated with adaptive intersexuality
Science, Oct 8, 2020; DOI: 10.1126/science.aaz2582

Exosome preparation

Author: Hidemasa Oh
Date: Dec 15, 2020，Views：828

Original protocol used in research article：

Cardiosphere-derived exosomal microRNAs for myocardial repair in pediatric dilated cardiomyopathy
Science Translational Medicine, Dec 8, 2020; DOI: 10.1126/scitranslmed.abb3336

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