Bio-101 - Improve Research Reproducibility

Restriction enzyme accessibility assay

Author: Sharon E Torigoe
Date: Dec 29, 2020，Views：1918

Original protocol used in research article：

ATP-dependent chromatin assembly is functionally distinct from chromatin remodeling
eLife, Aug 20, 2013; DOI: 10.7554/eLife.00863

Preparation of single nuclei suspension from adult Drosophila abdominal cuticle

Authors: Arpan C Ghosh and Norbert Perrimon
Date: Dec 28, 2020，Views：1103

Original protocol used in research article：

Drosophila PDGF/VEGF signaling from muscles to hepatocyte-like cells protects against obesity
eLife, Oct 27, 2020; DOI: 10.7554/eLife.56969

In vitro calcium imaging

Authors: Qiping Dong and Qiang Chang
Date: Dec 28, 2020，Views：1667

Original protocol used in research article：

Mechanism and consequence of abnormal calcium homeostasis in Rett syndrome astrocytes
eLife, Mar 29, 2018; DOI: 10.7554/eLife.33417

Constructs and stable cell lines

Author: Naeha Subramanian
Date: Dec 28, 2020，Views：955

Original protocol used in research article：

A small sustained increase in NOD1 abundance promotes ligand-independent inflammatory and oncogene transcriptional responses
Science Signaling, Dec 7, 2020; DOI: 10.1126/scisignal.aba3244

PBMC processing and stimulations

Authors: Thanaphong Phongpreecha, Rosemary Fernandez and Thomas Montine
Date: Dec 24, 2020，Views：1037

Original protocol used in research article：

Single-cell peripheral immunoprofiling of Alzheimer’s and Parkinson’s diseases
Science Advances, Nov 25, 2020; DOI: 10.1126/sciadv.abd5575

Lateral hypothalamus surgery

Author: Yeka Aponte
Date: Dec 22, 2020，Views：917

Original protocol used in research article：

High-throughput synapse-resolving two-photon fluorescence microendoscopy for deep-brain volumetric imaging in vivo
eLife, Jan 4, 2019; DOI: 10.7554/eLife.40805

In vitro cleavage assay and Electrophoretic Mobility Shift Assay (EMSA)

Author: Roger S. Zou
Date: Dec 22, 2020，Views：1025

Original protocol used in research article：

Very fast CRISPR on demand
Science, Jun 11, 2020; DOI: 10.1126/science.aay8204

TGIRT-seq library construction

Author: Alan M Lambowitz
Date: Dec 22, 2020，Views：1153

Original protocol used in research article：

Identification of protein-protected mRNA fragments and structured excised intron RNAs in human plasma by TGIRT-seq peak calling
eLife, Sep 2, 2020; DOI: 10.7554/eLife.60743

in vitro Fluorescence Resonance Energy Transfer (FRET) and FRET in cells

Authors: Seunghan Son and Chul Geun Kim
Date: Dec 22, 2020，Views：791

Original protocol used in research article：

Rational discovery of antimetastatic agents targeting the intrinsically disordered region of MBD2
Science Advances, Nov 19, 2019; DOI: 10.1126/sciadv.aav9810

Sequencing data processing and analysis

Authors: Alexandra Popa, Jakob-Wendelin Genger and Andreas Bergthaler
Date: Dec 22, 2020，Views：971

Original protocol used in research article：

Genomic epidemiology of superspreading events in Austria reveals mutational dynamics and transmission properties of SARS-CoV-2
Science Translational Medicine, Dec 9, 2020; DOI: 10.1126/scitranslmed.abe2555

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