Bio-101 - Improve Research Reproducibility

Detail Immunostaining Protocol for Pax6 mouse eye samples

Author: Ali R. Djalilian
Date: Dec 15, 2020，Views：769

Original protocol used in research article：

Gene dosage manipulation alleviates manifestations of hereditary PAX6 haploinsufficiency in mice
Science Translational Medicine, Dec 9, 2020; DOI: 10.1126/scitranslmed.aaz4894

Cell culture and reagents

Author: Melanie Girard
Date: Dec 15, 2020，Views：6066

Original protocol used in research article：

Type I interferons drive the maturation of human DC3s with a distinct costimulatory profile characterized by high GITRL
Science Immunology, Nov 12, 2020; DOI: 10.1126/sciimmunol.abe0347

Identification of species-specific expressed genes

Author: Jie Wang
Date: Dec 12, 2020，Views：6224

Original protocol used in research article：

Gene regulatory networks controlling vertebrate retinal regeneration
Science, Oct 1, 2020; DOI: 10.1126/science.abb8598

Pre-processing of fiber photometry signal

Author: Richard B Crouse
Date: Dec 11, 2020，Views：1012

Original protocol used in research article：

Acetylcholine is released in the basolateral amygdala in response to predictors of reward and enhances the learning of cue-reward contingency
eLife, Sep 18, 2020; DOI: 10.7554/eLife.57335

RNA constructs and in vitro transcription.

Author: Katharina Reinhard
Date: Dec 10, 2020，Views：977

Original protocol used in research article：

An RNA vaccine drives expansion and efficacy of claudin-CAR-T cells against solid tumors
Science, Jan 1, 2020; DOI: 10.1126/science.aay5967

STED imaging

Author: Monica M Sousa
Date: Dec 10, 2020，Views：847

Original protocol used in research article：

The membrane periodic skeleton is an actomyosin network that regulates axonal diameter and conduction
eLife, Mar 20, 2020; DOI: 10.7554/eLife.55471

Composite agarose-polyacrylamide gel electrophoresis (Composite AgPAGE)

Authors: Kirk Bergstrom, Xindi Shan and Lijun Xia
Date: Dec 9, 2020，Views：973

Original protocol used in research article：

Proximal colon–derived O-glycosylated mucus encapsulates and modulates the microbiota
Science, Oct 23, 2020; DOI: 10.1126/science.aay7367

Adoptive cell transfer (ACT) and RNA-LPX treatment

Author: Katharina Reinhard
Date: Dec 9, 2020，Views：1024

Original protocol used in research article：

An RNA vaccine drives expansion and efficacy of claudin-CAR-T cells against solid tumors
Science, Jan 1, 2020; DOI: 10.1126/science.aay5967

Ex vivo live imaging

Authors: Anamika Sharma and Gaiti Hasan
Date: Dec 9, 2020，Views：1136

Original protocol used in research article：

Modulation of flight and feeding behaviours requires presynaptic IP₃Rs in dopaminergic neurons
eLife, Nov 6, 2020; DOI: 10.7554/eLife.62297

Bacterial flow cytometry (BUGFlow)

Author: Anne-Katrin Pröbstel
Date: Dec 9, 2020，Views：1108

Original protocol used in research article：

Gut microbiota\–specific IgA+ B cells traffic to the CNS in active multiple sclerosis
Science Immunology, Nov 19, 2020; DOI: 10.1126/sciimmunol.abc7191

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