Bio-101 - Improve Research Reproducibility

16S rRNA gene analysis

Author: Xiaoyuan Zhou
Date: Dec 9, 2020，Views：877

Original protocol used in research article：

Gut microbiota\–specific IgA+ B cells traffic to the CNS in active multiple sclerosis
Science Immunology, Nov 19, 2020; DOI: 10.1126/sciimmunol.abc7191

LNP preparation

Authors: Anna Gutkin and Daniel Rosenblum
Date: Dec 8, 2020，Views：2221

Original protocol used in research article：

CRISPR-Cas9 genome editing using targeted lipid nanoparticles for cancer therapy
Science Advances, Nov 18, 2020; DOI: 10.1126/sciadv.abc9450

LNP transfection

Authors: Dan Peer, Anna Gutkin and Daniel Rosenblum
Date: Dec 8, 2020，Views：2630

Original protocol used in research article：

CRISPR-Cas9 genome editing using targeted lipid nanoparticles for cancer therapy
Science Advances, Nov 18, 2020; DOI: 10.1126/sciadv.abc9450

Phenotyping of adoptively transferred and endogenous tumor-infiltrating CD8+ T cells in the B16F10 orthotopic melanoma model

Authors: Meixi Hao, Caoyun Ju and Can Zhang
Date: Dec 8, 2020，Views：890

Original protocol used in research article：

Combination of metabolic intervention and T cell therapy enhances solid tumor immunotherapy
Science Translational Medicine, Nov 24, 2020; DOI: 10.1126/scitranslmed.aaz6667

Flow cytometry dissociation protocol for Individual Drosophila brains

Author: Shyama Nandakumar
Date: Dec 7, 2020，Views：1044

Original protocol used in research article：

Polyploidy in the adult Drosophila brain
eLife, Aug 25, 2020; DOI: 10.7554/eLife.54385

Evaluating T cell escape in epitopes previously defined as reactive in clade B consensus mapping - Protocol for Elispot, Peptide handling

Authors: Joanna A Warren and Nilu Goonetilleke
Date: Dec 7, 2020，Views：1010

Original protocol used in research article：

The HIV-1 latent reservoir is largely sensitive to circulating T cells
eLife, Oct 6, 2020; DOI: 10.7554/eLife.57246

Transfection, pool maintenance, and drug addition

Authors: Raghuvir Viswanatha and Norbert Perrimon
Date: Dec 7, 2020，Views：966

Original protocol used in research article：

Pooled genome-wide CRISPR screening for basal and context-specific fitness gene essentiality in Drosophila cells
eLife, Jul 27, 2018; DOI: 10.7554/eLife.36333

Stimuli

Author: Paolo Ossola
Date: Dec 7, 2020，Views：913

Original protocol used in research article：

Belief updating in bipolar disorder predicts time of recurrence
eLife, Nov 10, 2020; DOI: 10.7554/eLife.58891

Single-cell RNA-seq analysis of public 10x data

Authors: Jean-Baptiste Gorin, Vincent Wu, Son Nguyen and Marcus Buggert
Date: Dec 7, 2020，Views：840

Original protocol used in research article：

TOX is expressed by exhausted and polyfunctional human effector memory CD8+ T cells
Science Immunology, Jul 2, 2020; DOI: 10.1126/sciimmunol.aba7918

Isolation of single cells from cryoinjured hearts

Author: Jeroen Bakkers
Date: Dec 5, 2020，Views：897

Original protocol used in research article：

Single-cell analysis uncovers that metabolic reprogramming by ErbB2 signaling is essential for cardiomyocyte proliferation in the regenerating heart
eLife, Dec 23, 2019; DOI: 10.7554/eLife.50163

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