Bio-101 - Improve Research Reproducibility

Whole-mount Xenopus Immunostaining Protocol

Author: Andrea Elizabeth Wills
Date: Nov 11, 2020，Views：1010

Original protocol used in research article：

Chromatin accessibility dynamics and single cell RNA-Seq reveal new regulators of regeneration in neural progenitors
eLife, Apr 27, 2020; DOI: 10.7554/eLife.52648

Endosteal bone marrow Td+ cell isolation and cell sorting

Author: Leilei Zhong
Date: Nov 11, 2020，Views：1052

Original protocol used in research article：

Single cell transcriptomics identifies a unique adipose lineage cell population that regulates bone marrow environment
eLife, Apr 14, 2020; DOI: 10.7554/eLife.54695

Peripheral nervous system (PNS) axotomy assay

Authors: Sean D Speese and Mary A Logan
Date: Nov 10, 2020，Views：1128

Original protocol used in research article：

A novel Drosophila injury model reveals severed axons are cleared through a Draper/MMP-1 signaling cascade
eLife, Aug 21, 2017; DOI: 10.7554/eLife.23611

TRACE-seq Data analysis

Authors: Bo Lu, Liting Dong, Danyang Yi, Meiling Zhang, Chenxu Zhu, Xiaoyu Li and Chengqi Yi
Date: Nov 10, 2020，Views：1019

Original protocol used in research article：

Transposase-assisted tagmentation of RNA/DNA hybrid duplexes
eLife, Jul 23, 2020; DOI: 10.7554/eLife.54919

Preparation of monobody mRNA libraries for selection against SARS-CoV-2 targets

Authors: Taishi Kondo and Hiroshi Murakami
Date: Nov 9, 2020，Views：1123

Original protocol used in research article：

Antibody-like proteins that capture and neutralize SARS-CoV-2
Science Advances, Oct 14, 2020; DOI: 10.1126/sciadv.abd3916

Protocols for FACS purification of cardiac total interstitial single cells from adult mouse cardiac ventricles

Authors: Nona Farbehi, Vaibhao Janbandhu, Ralph Patrick, Munira Xaymardan, Robert R Nordon and Richard P Harvey
Date: Nov 9, 2020，Views：1192

Original protocol used in research article：

Single-cell expression profiling reveals dynamic flux of cardiac stromal, vascular and immune cells in health and injury
eLife, Mar 26, 2019; DOI: 10.7554/eLife.43882

rRNA depletion RNAseq protocol

Author: Ye Duan
Date: Nov 9, 2020，Views：1007

Original protocol used in research article：

RNA-seq with RNase H-based ribosomal RNA depletion specifically designed for C. elegans
microPublication Biology, Sep 22, 2020; DOI: 10.17912/micropub.biology.000312

Isolation, culture and identification of primary Müller cells from the macula and peripheral human retina

Authors: Ting Zhang, Yingying Chen, Shaoxue Zeng and Ling Zhu
Date: Nov 8, 2020，Views：1176

Original protocol used in research article：

Human macular Müller cells rely more on serine biosynthesis to combat oxidative stress than those from the periphery
eLife, Apr 30, 2019; DOI: 10.7554/eLife.43598

Optogenetic stimulation

Author: Shi-Bin Li
Date: Nov 7, 2020，Views：939

Original protocol used in research article：

Hypothalamic circuitry underlying stress-induced insomnia and peripheral immunosuppression
Science Advances, Sep 9, 2020; DOI: 10.1126/sciadv.abc2590

Chromatin immunoprecipitation (ChIP) protocol for the sponge Amphimedon queenslandica

Authors: Federico Gaiti, Bernard M Degnan and Miloš Tanurdžić
Date: Nov 7, 2020，Views：847

Original protocol used in research article：

Landscape of histone modifications in a sponge reveals the origin of animal cis-regulatory complexity
eLife, Apr 11, 2017; DOI: 10.7554/eLife.22194

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