Bio-101 - Improve Research Reproducibility

Immunostaining, hair cell and synaptic counts

Author: Guoqiang Wan
Date: Oct 26, 2020，Views：919

Original protocol used in research article：

Neurotrophin-3 regulates ribbon synapse density in the cochlea and induces synapse regeneration after acoustic trauma
eLife, Oct 20, 2014; DOI: 10.7554/eLife.03564

Assessment of CpG methylation status by bisulfite sequencing

Author: Yijing Su
Date: Oct 23, 2020，Views：876

Original protocol used in research article：

DNA methylation presents distinct binding sites for human transcription factors
eLife, Sep 3, 2013; DOI: 10.7554/eLife.00726

Brain tissue preparation and immunohistochemistry

Authors: Renato Socodato, Teresa Summavielle and João B. Relvas
Date: Oct 23, 2020，Views：931

Original protocol used in research article：

Daily alcohol intake triggers aberrant synaptic pruning leading to synapse loss and anxiety-like behavior
Science Signaling, Sep 21, 2020; DOI: 10.1126/scisignal.aba5754

Measurement of root growth and cell death area in the root tip using Arabidopsis seedlings that grow under DNA stress

Authors: Naoki Takahashi and Masaaki Umeda
Date: Oct 23, 2020，Views：1038

Original protocol used in research article：

A regulatory module controlling stress-induced cell cycle arrest in Arabidopsis
eLife, Apr 4, 2019; DOI: 10.7554/eLife.43944

Fluorescamine-based assay

Authors: Yiwei Yang, Falin Tian, Di Nie, Yuan Liu, Kun Qian, Miaorong Yu, Aohua Wang, Yaqi Zhang, Xinghua Shi and Yong Gan
Date: Oct 22, 2020，Views：822

Original protocol used in research article：

Rapid transport of germ-mimetic nanoparticles with dual conformational polyethylene glycol chains in biological tissues
Science Advances, Feb 6, 2020; DOI: 10.1126/sciadv.aay9937

Dose response curve fitting

Author: Ramnik J Xavier
Date: Oct 21, 2020，Views：974

Original protocol used in research article：

The kinase DYRK1A reciprocally regulates the differentiation of Th17 and regulatory T cells
eLife, May 22, 2015; DOI: 10.7554/eLife.05920

Cloning and purification of VapB22, VapC22, and VapBC22

Authors: Krishan Gopal Thakur and Ramandeep Singh
Date: Oct 21, 2020，Views：844

Original protocol used in research article：

VapBC22 toxin-antitoxin system from Mycobacterium tuberculosis is required for pathogenesis and modulation of host immune response
Science Advances, Jun 2, 2020; DOI: 10.1126/sciadv.aba6944

Electrophysiology

Authors: Shangbang Gao and Zhitao Hu
Date: Oct 21, 2020，Views：842

Original protocol used in research article：

Minimum number of synaptic vesicles for the initiation of a single action potential at C. elegans neuromuscular junction
microPublication Biology, Oct 6, 2020; DOI: 10.17912/micropub.biology.000316

Cell preparation for analysis

Author: Alok Kumar
Date: Oct 20, 2020，Views：928

Original protocol used in research article：

Tumors attenuating the mitochondrial activity in T cells escape from PD-1 blockade therapy
eLife, Mar 3, 2020; DOI: 10.7554/eLife.52330

Quantification of the diversities of V(D)J recombination events for a given CDR3 peptide

Authors: Jian Han and Leonore A Herzenberg
Date: Oct 20, 2020，Views：959

Original protocol used in research article：

Distinct mechanisms define murine B cell lineage immunoglobulin heavy chain (IgH) repertoires
eLife, Sep 30, 2015; DOI: 10.7554/eLife.09083

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