Bio-101 - Improve Research Reproducibility

Pathogen inoculation procedure

Author: Shouan Liu
Date: Oct 27, 2020，Views：1310

Original protocol used in research article：

Negative regulation of ABA signaling by WRKY33 is critical for Arabidopsis immunity towards Botrytis cinerea 2100
eLife, Jun 15, 2015; DOI: 10.7554/eLife.07295

Purification

Author: Jia-Yin Tsai
Date: Oct 27, 2020，Views：822

Original protocol used in research article：

Structure of the sodium-dependent phosphate transporter reveals insights into human solute carrier SLC20
Science Advances, Aug 6, 2020; DOI: 10.1126/sciadv.abb4024

Imaging of microdroplets and image analysis

Author: SangMoon Lhee
Date: Oct 27, 2020，Views：788

Original protocol used in research article：

Spatial localization of charged molecules by salt ions in oil-confined water microdroplets
Science Advances, Oct 6, 2020; DOI: 10.1126/sciadv.aba0181

Neoangiogenesis

Authors: D. A. Golombek and Natalia Paladino
Date: Oct 27, 2020，Views：859

Original protocol used in research article：

Circadian disruption promotes tumor-immune microenvironment remodeling favoring tumor cell proliferation
Science Advances, Oct 14, 2020; DOI: 10.1126/sciadv.aaz4530

Fission yeast strains and general techniques

Authors: Tomoyuki Fukuda and Kazuhiro Shiozaki
Date: Oct 27, 2020，Views：808

Original protocol used in research article：

Ragulator and GATOR1 complexes promote fission yeast growth by attenuating TOR complex 1 through Rag GTPases
eLife, Dec 4, 2017; DOI: 10.7554/eLife.30880

Tissue preparation

Author: Pamela Maher
Date: Oct 27, 2020，Views：1027

Original protocol used in research article：

Elevating acetyl-CoA levels reduces aspects of brain aging
eLife, Nov 19, 2019; DOI: 10.7554/eLife.47866

Immunofluorescence and immunohistochemistry

Author: Emanuele Puca
Date: Oct 27, 2020，Views：814

Original protocol used in research article：

Immunocytokines are a promising immunotherapeutic approach against glioblastoma
Science Translational Medicine, Oct 6, 2020; DOI: 10.1126/scitranslmed.abb2311

Genetic manipulation of S. aureus mutants

Authors: Katarzyna Wacnik and Simon J Foster
Date: Oct 27, 2020，Views：2333

Original protocol used in research article：

Molecular coordination of Staphylococcus aureus cell division
eLife, Feb 21, 2018; DOI: 10.7554/eLife.32057

LC3 lipidation, membrane fractionation, immunodepletion, and immunoblot

Author: Liang Ge
Date: Oct 26, 2020，Views：1042

Original protocol used in research article：

Phosphatidylinositol 3-kinase and COPII generate LC3 lipidation vesicles from the ER-Golgi intermediate compartment
eLife, Nov 28, 2014; DOI: 10.7554/eLife.04135

Animal care and stress paradigm

Author: Jaideep Singh Bains
Date: Oct 26, 2020，Views：1129

Original protocol used in research article：

Sexually dimorphic neuronal responses to social isolation
eLife, Oct 11, 2016; DOI: 10.7554/eLife.18726

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