Bio-101 - Improve Research Reproducibility

CRISPR-Cas9 sgRNA design and construction

Author: Yen-Lin Huang
Date: Nov 3, 2020，Views：2054

Original protocol used in research article：

Collagen-rich omentum is a premetastatic niche for integrin α2-mediated peritoneal metastasis
eLife, Oct 7, 2020; DOI: 10.7554/eLife.59442

Hfq UV cross-linking, ligation and analysis of hybrids (Hfq-CLASH)

Authors: Stuart William Mckellar, Ira Alexandra Iosub, Ivayla Ivanova and Sander Granneman
Date: Nov 3, 2020，Views：914

Original protocol used in research article：

Hfq CLASH uncovers sRNA-target interaction networks linked to nutrient availability adaptation
eLife, May 1, 2020; DOI: 10.7554/eLife.54655

Generation of Gadl1-null mice

Author: Elaheh Mahootchi
Date: Nov 3, 2020，Views：816

Original protocol used in research article：

GADL1 is a multifunctional decarboxylase with tissue-specific roles in β-alanine and carnosine production
Science Advances, Jul 16, 2020; DOI: 10.1126/sciadv.abb3713

ssDRIP-seq and DRIP-qPCR

Authors: Yaoyi Li, Wei Xu, Yawei Song, Qin Li, Qianwen Sun and Hongjie Yao
Date: Nov 2, 2020，Views：1256

Original protocol used in research article：

R-loops coordinate with SOX2 in regulating reprogramming to pluripotency
Science Advances, Jun 9, 2020; DOI: 10.1126/sciadv.aba0777

2P-seq

Authors: Yunkun Dang, Yi Liu and Zhipeng Zhou
Date: Nov 2, 2020，Views：918

Original protocol used in research article：

Codon usage biases co-evolve with transcription termination machinery to suppress premature cleavage and polyadenylation
eLife, Mar 16, 2018; DOI: 10.7554/eLife.33569

Live Cell Imaging of EGF Binding to A431 Cells

Authors: Dongmyung Oh, Joshua A Jadwin, Bruce J Mayer and Ji Yu
Date: Nov 2, 2020，Views：870

Original protocol used in research article：

Time-resolved multimodal analysis of Src Homology 2 (SH2) domain binding in signaling by receptor tyrosine kinases
eLife, Apr 13, 2016; DOI: 10.7554/eLife.11835

Isolation of synaptosomes and glutamate release assay

Author: Reinhard Jahn
Date: Nov 2, 2020，Views：832

Original protocol used in research article：

Analysis of protein phosphorylation in nerve terminal reveals extensive changes in active zone proteins upon exocytosis
eLife, Apr 26, 2016; DOI: 10.7554/eLife.14530

Western blotting

Author: Jonathan Houseley
Date: Nov 2, 2020，Views：934

Original protocol used in research article：

Tri-methylation of histone H3 lysine 4 facilitates gene expression in ageing cells
eLife, Oct 2, 2018; DOI: 10.7554/eLife.34081

Computation of evolutionary couplings

Author: Alexandre M J J Bonvin
Date: Nov 2, 2020，Views：951

Original protocol used in research article：

Sequence co-evolution gives 3D contacts and structures of protein complexes
eLife, Sep 25, 2014; DOI: 10.7554/eLife.03430

Demonstration of the Stimulatory Effect of Lipopolysaccharide (LPS) on Egg Laying in Caenorhabditis elegans

Authors: Angela Ching-Yee Leung and Myeongwoo Lee
Date: Nov 2, 2020，Views：775

Original protocol used in research article：

Lipopolysaccharide stimulates egg laying in Caenorhabditis elegans
microPublication Biology, Sep 14, 2020; DOI: 10.17912/micropub.biology.000308

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