Bio-101 - Improve Research Reproducibility

Generation of droplet-based artificial cells

Authors: Ye Guan, Shiyuan Wang and Qiong Yang
Date: Nov 2, 2020，Views：956

Original protocol used in research article：

A robust and tunable mitotic oscillator in artificial cells
eLife, Apr 5, 2018; DOI: 10.7554/eLife.33549

Golgi-cox staining, dendritic spine analysis and neuronal morphology

Author: W Christopher Risher
Date: Nov 2, 2020，Views：985

Original protocol used in research article：

Astrocytes refine cortical connectivity at dendritic spines
eLife, Dec 17, 2014; DOI: 10.7554/eLife.04047

In vitro efferocytosis

Authors: Arif Yurdagul Jr. and Ira Tabas
Date: Oct 31, 2020，Views：956

Original protocol used in research article：

siRNA nanoparticles targeting CaMKIIγ in lesional macrophages improve atherosclerotic plaque stability in mice
Science Translational Medicine, Jul 21, 2020; DOI: 10.1126/scitranslmed.aay1063

Statistical analysis

Authors: Cassondra L. Vernier and Yehuda Ben-Shahar
Date: Oct 30, 2020，Views：815

Original protocol used in research article：

The gut microbiome defines social group membership in honey bee colonies
Science Advances, Oct 14, 2020; DOI: 10.1126/sciadv.abd3431

Polysome and Puromycin immunoprecipitation

Authors: Benjamin D Hobson and Peter A Sims
Date: Oct 30, 2020，Views：1570

Original protocol used in research article：

Elongation inhibitors do not prevent the release of puromycylated nascent polypeptide chains from ribosomes
eLife, Aug 26, 2020; DOI: 10.7554/eLife.60048

Cell cycle prediction

Authors: Anneke Dixie Kakebeen and Andrea Elizabeth Wills
Date: Oct 29, 2020，Views：980

Original protocol used in research article：

Chromatin accessibility dynamics and single cell RNA-Seq reveal new regulators of regeneration in neural progenitors
eLife, Apr 27, 2020; DOI: 10.7554/eLife.52648

3D vascular imaging

Authors: Célian Bimbard, Charlie Demene and Yves Boubenec
Date: Oct 29, 2020，Views：856

Original protocol used in research article：

Multi-scale mapping along the auditory hierarchy using high-resolution functional UltraSound in the awake ferret
eLife, Jun 28, 2018; DOI: 10.7554/eLife.35028

List of antibodies used for cell sorting

Author: Jaromír Mikeš
Date: Oct 29, 2020，Views：807

Original protocol used in research article：

A genome-wide transcriptomic analysis of protein-coding genes in human blood cells
Science, Dec 19, 2019; DOI: 10.1126/science.aax9198

Compound treatment assay for C. elegans

Authors: Di Zhu and Ye Tian
Date: Oct 28, 2020，Views：867

Original protocol used in research article：

NuRD mediates mitochondrial stress–induced longevity via chromatin remodeling in response to acetyl-CoA level
Science Advances, Jul 30, 2020; DOI: 10.1126/sciadv.abb2529

In vitro collagenase activity assay

Authors: Honoo Satake and Shin Matsubara
Date: Oct 28, 2020，Views：1308

Original protocol used in research article：

The regulation of oocyte maturation and ovulation in the closest sister group of vertebrates
eLife, Oct 1, 2019; DOI: 10.7554/eLife.49062

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