Bio-101 - Improve Research Reproducibility

cLTP and cLTD protocols

Author: Okunola Jeyifous
Date: Jul 22, 2020，Views：1618

Original protocol used in research article：

Synaptic activity regulates AMPA receptor trafficking through different recycling pathways
eLife, May 13, 2015; DOI: 10.7554/eLife.06878

Block-bootstrap procedure

Author: Fanny Pouyet
Date: Jul 21, 2020，Views：1287

Original protocol used in research article：

Background selection and biased gene conversion affect more than 95% of the human genome and bias demographic inferences
eLife, Aug 23, 2018; DOI: 10.7554/eLife.36317

In vitro effects of YAP1-targeting drugs

Authors: Tomohiko Maehama, Akira Suzuki and Hirofumi Omori
Date: Jul 21, 2020，Views：890

Original protocol used in research article：

YAP1 is a potent driver of the onset and progression of oral squamous cell carcinoma
Science Advances, Mar 17, 2020; DOI: 10.1126/sciadv.aay3324

Overexpression and purification of the recombinant β-actin inclusion-body protein

Author: Jakub Drozak
Date: Jul 21, 2020，Views：1049

Original protocol used in research article：

Structural insights into SETD3-mediated histidine methylation on β-actin
eLife, Feb 20, 2019; DOI: 10.7554/eLife.43676

Calculation of residue-residue contact score (RRCS)

Authors: Qingtong Zhou, Ming-Wei Wang and Suwen Zhao
Date: Jul 21, 2020，Views：1504

Original protocol used in research article：

Common activation mechanism of class A GPCRs
eLife, Dec 19, 2019; DOI: 10.7554/eLife.50279

Expression and purification of SpyCas9

Authors: Xinyu Ling and Tao Liu
Date: Jul 20, 2020，Views：1052

Original protocol used in research article：

Improving the efficiency of precise genome editing with site-specific Cas9-oligonucleotide conjugates
Science Advances, Apr 7, 2020; DOI: 10.1126/sciadv.aaz0051

Phagocytosis assay

Authors: Alexandre B Leitão and Élio Sucena
Date: Jul 20, 2020，Views：980

Original protocol used in research article：

Drosophila sessile hemocyte clusters are true hematopoietic tissues that regulate larval blood cell differentiation
eLife, Feb 4, 2015; DOI: 10.7554/eLife.06166

Barcode association of library variants

Authors: Julia M Flynn and Daniel NA Bolon
Date: Jul 20, 2020，Views：1055

Original protocol used in research article：

Comprehensive fitness maps of Hsp90 show widespread environmental dependence
eLife, Mar 4, 2020; DOI: 10.7554/eLife.53810

Probability of acquisition

Author: Thomas Crellen
Date: Jul 20, 2020，Views：1118

Original protocol used in research article：

Transmission dynamics and control of multidrug-resistant Klebsiella pneumoniae in neonates in a developing country
eLife, Dec 3, 2019; DOI: 10.7554/eLife.50468

In vivo efficacy study

Authors: Chad V. Pecot, Alexander V. Kabanov and Natasha Vinod
Date: Jul 17, 2020，Views：918

Original protocol used in research article：

High-capacity poly(2-oxazoline) formulation of TLR 7/8 agonist extends survival in a chemo-insensitive metastatic model of lung adenocarcinoma
Science Advances, Jun 16, 2020; DOI: 10.1126/sciadv.aba5542

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