Bio-101 - Improve Research Reproducibility

X34 staining

Author: Della C David
Date: May 26, 2020，Views：2396

Original protocol used in research article：

Intrinsically aggregation-prone proteins form amyloid-like aggregates and contribute to tissue aging in Caenorhabditis elegans
eLife, May 3, 2019; DOI: 10.7554/eLife.43059

Protein network analysis

Author: Miguel A. Gonzalez-Lozano
Date: May 25, 2020，Views：933

Original protocol used in research article：

Stitching the synapse: Cross-linking mass spectrometry into resolving synaptic protein interactions
Science Advances, Feb 19, 2020; DOI: 10.1126/sciadv.aax5783

Cell viability assays following treatment with aDT–eIF-3b

Author: Laura Smith
Date: May 24, 2020，Views：929

Original protocol used in research article：

Attenuated diphtheria toxin mediates siRNA delivery
Science Advances, Apr 30, 2020; DOI: 10.1126/sciadv.aaz4848

Tau expression and purification

Authors: Wenjuan Zhang, Michel Goedert and Sjors HW Scheres
Date: May 24, 2020，Views：6294

Original protocol used in research article：

Heparin-induced tau filaments are polymorphic and differ from those in Alzheimer’s and Pick’s diseases
eLife, Feb 5, 2019; DOI: 10.7554/eLife.43584

Immunohistochemistry (IHC) of DRG

Author: Diana M Bautista
Date: May 23, 2020，Views：3172

Original protocol used in research article：

The signaling lipid sphingosine 1-phosphate regulates mechanical pain
eLife, Mar 21, 2018; DOI: 10.7554/eLife.33285

hiPSC maintenance and differentiation

Authors: Swagata Ghatak, Dorit Trudler, Nima Dolatabadi and Stuart A Lipton
Date: May 22, 2020，Views：1289

Original protocol used in research article：

Mechanisms of hyperexcitability in Alzheimer’s disease hiPSC-derived neurons and cerebral organoids vs isogenic controls
eLife, Nov 29, 2019; DOI: 10.7554/eLife.50333

Genus-level phylogenetic tree

Author: Ricardo A. Segovia
Date: May 22, 2020，Views：1046

Original protocol used in research article：

Freezing and water availability structure the evolutionary diversity of trees across the Americas
Science Advances, May 5, 2020; DOI: 10.1126/sciadv.aaz5373

AcrIIA11 homolog discovery and phylogenetic placement

Author: Kevin J Forsberg
Date: May 21, 2020，Views：1147

Original protocol used in research article：

Functional metagenomics-guided discovery of potent Cas9 inhibitors in the human microbiome
eLife, Sep 10, 2019; DOI: 10.7554/eLife.46540

Synthesis of compounds

Author: Samuel T. Jones
Date: May 20, 2020，Views：972

Original protocol used in research article：

Modified cyclodextrins as broad-spectrum antivirals
Science Advances, Jan 28, 2020; DOI: 10.1126/sciadv.aax9318

Golgi preparations

Authors: Alun M Davies and Paulina Carriba
Date: May 20, 2020，Views：1024

Original protocol used in research article：

CD40 is a major regulator of dendrite growth from developing excitatory and inhibitory neurons
eLife, Nov 7, 2017; DOI: 10.7554/eLife.30442

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