Protocols shared by authors via RaP service
Journal
ChIP coupled to quantitative RT-PCR (qRT-PCR)
Author: Joseph Strauss
Date: Jul 10, 2020,Views:1020
Original protocol used in research article:
Chromatin mapping identifies BasR, a key regulator of bacteria-triggered production of fungal secondary metabolites
eLife, Oct 12, 2018; DOI: 10.7554/eLife.40969
Processing of single-cell data
Author: Evan Witt
Date: Jul 10, 2020,Views:1060
Original protocol used in research article:
Testis single-cell RNA-seq reveals the dynamics of de novo gene transcription and germline mutational bias in Drosophila
eLife, Aug 16, 2019; DOI: 10.7554/eLife.47138
Analyses of individual genes
Author: Fanny Pouyet
Date: Jul 8, 2020,Views:1028
Original protocol used in research article:
Recombination, meiotic expression and human codon usage
eLife, Aug 15, 2017; DOI: 10.7554/eLife.27344
O2 release from PCC7942
Author: Huanhuan Chen
Date: Jul 7, 2020,Views:1092
Original protocol used in research article:
Dissolved oxygen from microalgae-gel patch promotes chronic wound healing in diabetes
Science Advances, May 14, 2020; DOI: 10.1126/sciadv.aba4311
Viral-mediated gene delivery for in vivo circuit manipulation in neonatal mice
Authors: Yasunobu Murata and Matthew T Colonnese
Date: Jul 6, 2020,Views:1107
Original protocol used in research article:
GABAergic interneurons excite neonatal hippocampus in vivo
Science Advances, Jun 11, 2020; DOI: 10.1126/sciadv.aba1430
Glutaraldehyde Cross-linked Nun/TEC Preparation for CryoEM
Authors: Jin Young Kang and Seth A Darst
Date: Jul 6, 2020,Views:1196
Original protocol used in research article:
Structural basis of transcription arrest by coliphage HK022 Nun in an Escherichia coli RNA polymerase elongation complex
eLife, Mar 20, 2017; DOI: 10.7554/eLife.25478
Glutaraldehyde Cross-linked TEC preparation for CryoEM
Authors: Jin Young Kang and Seth A Darst
Date: Jul 5, 2020,Views:1411
Original protocol used in research article:
Structural basis of transcription arrest by coliphage HK022 Nun in an Escherichia coli RNA polymerase elongation complex
eLife, Mar 20, 2017; DOI: 10.7554/eLife.25478
Implementing CILP
Author: Amanda Lea
Date: Jul 5, 2020,Views:1024
Original protocol used in research article:
Genetic and environmental perturbations lead to regulatory decoherence
eLife, Mar 5, 2019; DOI: 10.7554/eLife.40538
Pulldown assay
Author: Richard T Lee
Date: Jul 5, 2020,Views:1183
Original protocol used in research article:
Diabetes regulates fructose absorption through thioredoxin-interacting protein
eLife, Oct 11, 2016; DOI: 10.7554/eLife.18313
Cell culture, nuclei isolation and GRO-seq library preparation
Authors: Iris Jonkers and John T Lis
Date: Jul 5, 2020,Views:1188
Original protocol used in research article:
Genome-wide dynamics of Pol II elongation and its interplay with promoter proximal pausing, chromatin, and exons
eLife, Apr 29, 2014; DOI: 10.7554/eLife.02407