Bio-101 - Improve Research Reproducibility

ChIP coupled to quantitative RT-PCR (qRT-PCR)

Author: Joseph Strauss
Date: Jul 10, 2020，Views：1020

Original protocol used in research article：

Chromatin mapping identifies BasR, a key regulator of bacteria-triggered production of fungal secondary metabolites
eLife, Oct 12, 2018; DOI: 10.7554/eLife.40969

Processing of single-cell data

Author: Evan Witt
Date: Jul 10, 2020，Views：1060

Original protocol used in research article：

Testis single-cell RNA-seq reveals the dynamics of de novo gene transcription and germline mutational bias in Drosophila
eLife, Aug 16, 2019; DOI: 10.7554/eLife.47138

Analyses of individual genes

Author: Fanny Pouyet
Date: Jul 8, 2020，Views：1028

Original protocol used in research article：

Recombination, meiotic expression and human codon usage
eLife, Aug 15, 2017; DOI: 10.7554/eLife.27344

O2 release from PCC7942

Author: Huanhuan Chen
Date: Jul 7, 2020，Views：1092

Original protocol used in research article：

Dissolved oxygen from microalgae-gel patch promotes chronic wound healing in diabetes
Science Advances, May 14, 2020; DOI: 10.1126/sciadv.aba4311

Viral-mediated gene delivery for in vivo circuit manipulation in neonatal mice

Authors: Yasunobu Murata and Matthew T Colonnese
Date: Jul 6, 2020，Views：1107

Original protocol used in research article：

GABAergic interneurons excite neonatal hippocampus in vivo
Science Advances, Jun 11, 2020; DOI: 10.1126/sciadv.aba1430

Glutaraldehyde Cross-linked Nun/TEC Preparation for CryoEM

Authors: Jin Young Kang and Seth A Darst
Date: Jul 6, 2020，Views：1196

Original protocol used in research article：

Structural basis of transcription arrest by coliphage HK022 Nun in an Escherichia coli RNA polymerase elongation complex
eLife, Mar 20, 2017; DOI: 10.7554/eLife.25478

Glutaraldehyde Cross-linked TEC preparation for CryoEM

Authors: Jin Young Kang and Seth A Darst
Date: Jul 5, 2020，Views：1411

Original protocol used in research article：

Structural basis of transcription arrest by coliphage HK022 Nun in an Escherichia coli RNA polymerase elongation complex
eLife, Mar 20, 2017; DOI: 10.7554/eLife.25478

Implementing CILP

Author: Amanda Lea
Date: Jul 5, 2020，Views：1024

Original protocol used in research article：

Genetic and environmental perturbations lead to regulatory decoherence
eLife, Mar 5, 2019; DOI: 10.7554/eLife.40538

Pulldown assay

Author: Richard T Lee
Date: Jul 5, 2020，Views：1183

Original protocol used in research article：

Diabetes regulates fructose absorption through thioredoxin-interacting protein
eLife, Oct 11, 2016; DOI: 10.7554/eLife.18313

Cell culture, nuclei isolation and GRO-seq library preparation

Authors: Iris Jonkers and John T Lis
Date: Jul 5, 2020，Views：1188

Original protocol used in research article：

Genome-wide dynamics of Pol II elongation and its interplay with promoter proximal pausing, chromatin, and exons
eLife, Apr 29, 2014; DOI: 10.7554/eLife.02407

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