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A high-quality database for basic life science protocols
实验方法
研究领域
癌症生物学 (420)
发育生物学 (311)
分子生物学 (1101)
干细胞 (243)
免疫学 (584)
神经科学 (676)
生物化学 (1346)
生物物理学 (156)
微生物学 (1134)
系统生物学 (322)
细胞生物学 (2063)
植物科学 (1036)
Bio-protocol 期刊
Bio-101 实验手册
课题组实验方法合辑
Request a Protocol
提交
提交实验方法
作者指南
关于
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Biochemical and immunohistological characterization of brain tissue
Authors:
Stefan F Lichtenthaler
and
Sabina Tahirovic
Date: Jul 28, 2020,Views:962
Original protocol used in research article:
Fibrillar Aβ triggers microglial proteome alterations and dysfunction in Alzheimer mouse models
eLife, Jun 8, 2020; DOI:
10.7554/eLife.54083
Reverse phase(RP)-high-performance liquid chromatography (HPLC) analysis of globin chains
Authors:
Anne Chalumeau
,
Giacomo Frati
and
Annarita Miccio
Date: Jul 28, 2020,Views:1110
Original protocol used in research article:
Editing a γ-globin repressor binding site restores fetal hemoglobin synthesis and corrects the sickle cell disease phenotype
Science Advances, Feb 12, 2020; DOI:
10.1126/sciadv.aay9392
In vivo biodistribution of AUNPs after PDT
Author:
Duo Mao
Date: Jul 28, 2020,Views:1038
Original protocol used in research article:
AIEgen-coupled upconversion nanoparticles eradicate solid tumors through dual-mode ROS activation
Science Advances, Jun 25, 2020; DOI:
10.1126/sciadv.abb2712
Insects and UV images
Author:
Ryo Futahashi
Date: Jul 27, 2020,Views:1005
Original protocol used in research article:
Molecular basis of wax-based color change and UV reflection in dragonflies
eLife, Jan 15, 2019; DOI:
10.7554/eLife.43045
Immunohistochemistry
Authors:
Joel K Elmquist
,
Alexandre Caron
and
Charlotte Elizabeth Lee
Date: Jul 27, 2020,Views:1087
Original protocol used in research article:
POMC neurons expressing leptin receptors coordinate metabolic responses to fasting via suppression of leptin levels
eLife, Mar 12, 2018; DOI:
10.7554/eLife.33710
Real-time quantitative PCR
Author:
Sunhye Shin
Date: Jul 27, 2020,Views:1113
Original protocol used in research article:
Dynamic control of adipose tissue development and adult tissue homeostasis by platelet-derived growth factor receptor alpha
eLife, Jun 19, 2020; DOI:
10.7554/eLife.56189
Experiment 1: Effect of central (ICV) administration of senktide on LH release in male and female WT and Kiss1 KO mice with or without the presence of sex steroids.
Author:
Chrysanthi Fergani
Date: Jul 27, 2020,Views:948
Original protocol used in research article:
NKB signaling in the posterodorsal medial amygdala stimulates gonadotropin release in a kisspeptin-independent manner in female mice
eLife, Dec 19, 2018; DOI:
10.7554/eLife.40476
Generation of knockout flies
Author:
Mohammed A. Khallaf
Date: Jul 27, 2020,Views:1010
Original protocol used in research article:
Mate discrimination among subspecies through a conserved olfactory pathway
Science Advances, Jun 16, 2020; DOI:
10.1126/sciadv.aba5279
Chromatin immunoprecipitation-deep sequencing (ChIP-seq) and ChIP-qPCR
Authors:
Dong Fang
and
Zhiguo Zhang
Date: Jul 26, 2020,Views:3200
Original protocol used in research article:
H3.3K27M mutant proteins reprogram epigenome by sequestering the PRC2 complex to poised enhancers
eLife, Jun 22, 2018; DOI:
10.7554/eLife.36696
Human embryonic lung organoid culture including recovering organoids from matrigel for immunostaining or RNA extraction
Authors:
Emma L Rawlins
and
Dawei Sun
Date: Jul 23, 2020,Views:1296
Original protocol used in research article:
Human embryonic lung epithelial tips are multipotent progenitors that can be expanded in vitro as long-term self-renewing organoids
eLife, Jun 30, 2017; DOI:
10.7554/eLife.26575
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