Bio-101 - Improve Research Reproducibility

Biochemical and immunohistological characterization of brain tissue

Authors: Stefan F Lichtenthaler and Sabina Tahirovic
Date: Jul 28, 2020，Views：962

Original protocol used in research article：

Fibrillar Aβ triggers microglial proteome alterations and dysfunction in Alzheimer mouse models
eLife, Jun 8, 2020; DOI: 10.7554/eLife.54083

Reverse phase(RP)-high-performance liquid chromatography (HPLC) analysis of globin chains

Authors: Anne Chalumeau, Giacomo Frati and Annarita Miccio
Date: Jul 28, 2020，Views：1110

Original protocol used in research article：

Editing a γ-globin repressor binding site restores fetal hemoglobin synthesis and corrects the sickle cell disease phenotype
Science Advances, Feb 12, 2020; DOI: 10.1126/sciadv.aay9392

In vivo biodistribution of AUNPs after PDT

Author: Duo Mao
Date: Jul 28, 2020，Views：1038

Original protocol used in research article：

AIEgen-coupled upconversion nanoparticles eradicate solid tumors through dual-mode ROS activation
Science Advances, Jun 25, 2020; DOI: 10.1126/sciadv.abb2712

Insects and UV images

Author: Ryo Futahashi
Date: Jul 27, 2020，Views：1005

Original protocol used in research article：

Molecular basis of wax-based color change and UV reflection in dragonflies
eLife, Jan 15, 2019; DOI: 10.7554/eLife.43045

Immunohistochemistry

Authors: Joel K Elmquist, Alexandre Caron and Charlotte Elizabeth Lee
Date: Jul 27, 2020，Views：1087

Original protocol used in research article：

POMC neurons expressing leptin receptors coordinate metabolic responses to fasting via suppression of leptin levels
eLife, Mar 12, 2018; DOI: 10.7554/eLife.33710

Real-time quantitative PCR

Author: Sunhye Shin
Date: Jul 27, 2020，Views：1113

Original protocol used in research article：

Dynamic control of adipose tissue development and adult tissue homeostasis by platelet-derived growth factor receptor alpha
eLife, Jun 19, 2020; DOI: 10.7554/eLife.56189

Experiment 1: Effect of central (ICV) administration of senktide on LH release in male and female WT and Kiss1 KO mice with or without the presence of sex steroids.

Author: Chrysanthi Fergani
Date: Jul 27, 2020，Views：948

Original protocol used in research article：

NKB signaling in the posterodorsal medial amygdala stimulates gonadotropin release in a kisspeptin-independent manner in female mice
eLife, Dec 19, 2018; DOI: 10.7554/eLife.40476

Generation of knockout flies

Author: Mohammed A. Khallaf
Date: Jul 27, 2020，Views：1010

Original protocol used in research article：

Mate discrimination among subspecies through a conserved olfactory pathway
Science Advances, Jun 16, 2020; DOI: 10.1126/sciadv.aba5279

Chromatin immunoprecipitation-deep sequencing (ChIP-seq) and ChIP-qPCR

Authors: Dong Fang and Zhiguo Zhang
Date: Jul 26, 2020，Views：3200

Original protocol used in research article：

H3.3K27M mutant proteins reprogram epigenome by sequestering the PRC2 complex to poised enhancers
eLife, Jun 22, 2018; DOI: 10.7554/eLife.36696

Human embryonic lung organoid culture including recovering organoids from matrigel for immunostaining or RNA extraction

Authors: Emma L Rawlins and Dawei Sun
Date: Jul 23, 2020，Views：1296

Original protocol used in research article：

Human embryonic lung epithelial tips are multipotent progenitors that can be expanded in vitro as long-term self-renewing organoids
eLife, Jun 30, 2017; DOI: 10.7554/eLife.26575

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