Bio-101 - Improve Research Reproducibility

KEGG pathway enrichment analysis using clusterProfiler

Authors: Manon Morin, Emily C Pierce and Rachel J Dutton
Date: Aug 4, 2020，Views：1432

Original protocol used in research article：

Changes in the genetic requirements for microbial interactions with increasing community complexity
eLife, Sep 13, 2018; DOI: 10.7554/eLife.37072

Connectomic Analysis of Tissues

Author: Sam Brickman Raredon
Date: Aug 3, 2020，Views：930

Original protocol used in research article：

Single-cell connectomic analysis of adult mammalian lungs
Science Advances, Dec 3, 2019; DOI: 10.1126/sciadv.aaw3851

Immunohistochemistry on whole mount retinas

Author: Claudia Prahst
Date: Aug 3, 2020，Views：1770

Original protocol used in research article：

Mouse retinal cell behaviour in space and time using light sheet fluorescence microscopy
eLife, Feb 19, 2020; DOI: 10.7554/eLife.49779

C-RNA Sample Preparation Protocol

Authors: Carlo Randise-Hinchliff and Sarah Munchel
Date: Aug 2, 2020，Views：1041

Original protocol used in research article：

Circulating transcripts in maternal blood reflect a molecular signature of early-onset preeclampsia
Science Translational Medicine, Jun 30, 2020; DOI: 10.1126/scitranslmed.aaz0131

Single-cell intracellular Ca2+ microfluorimetry

Author: Arthur Conigrave
Date: Aug 1, 2020，Views：1021

Original protocol used in research article：

Structural mechanism of ligand activation in human calcium-sensing receptor
eLife, Jul 19, 2016; DOI: 10.7554/eLife.13662

Flow cytometry and cell sorting

Authors: Andreas Weigert and Rajkumar Savai
Date: Aug 1, 2020，Views：971

Original protocol used in research article：

Reprogramming of tumor-associated macrophages by targeting β-catenin/FOSL2/ARID5A signaling: A potential treatment of lung cancer
Science Advances, Jun 4, 2020; DOI: 10.1126/sciadv.aaz6105

Gene set enrichment analysis (GSEA)

Authors: Alexander J. Tarashansky and Bo Wang
Date: Jul 31, 2020，Views：1015

Original protocol used in research article：

Self-assembling manifolds in single-cell RNA sequencing data
eLife, Sep 16, 2019; DOI: 10.7554/eLife.48994

ATPase assay

Authors: Jesper Levring and Ling Wang
Date: Jul 30, 2020，Views：1216

Original protocol used in research article：

Characterization of the kinetic cycle of an ABC transporter by single-molecule and cryo-EM analyses
eLife, May 27, 2020; DOI: 10.7554/eLife.56451

Intracellular IRF5 Dimerization Assay

Authors: Cherrie D Sherman and Betsy J Barnes
Date: Jul 29, 2020，Views：7776

Original protocol used in research article：

Inhibition of IRF5 cellular activity with cell-penetrating peptides that target homodimerization
Science Advances, May 14, 2020; DOI: 10.1126/sciadv.aay1057

Construction of transgenic, knockout and knockin lines of drosophila

Author: Juan Huang
Date: Jul 29, 2020，Views：1064

Original protocol used in research article：

Sleep homeostasis regulated by 5HT2b receptor in a small subset of neurons in the dorsal fan-shaped body of drosophila
eLife, Oct 6, 2017; DOI: 10.7554/eLife.26519

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