Bio-101 - Improve Research Reproducibility

Protein expression and purification

Authors: Si Liu and Jiangtao Guo
Date: Sep 14, 2020，Views：1182

Original protocol used in research article：

Cryo-EM structures of the human cation-chloride cotransporter KCC1
Science, Oct 25, 2019; DOI: 10.1126/science.aay3129

Human monocyte-derived dendritic cell (moDC) differentiation and suppression of moDCs by Tregs

Authors: Qing Jessica Huang and Megan K. Levings
Date: Sep 13, 2020，Views：1070

Original protocol used in research article：

Functional effects of chimeric antigen receptor co-receptor signaling domains in human regulatory T cells
Science Translational Medicine, Aug 19, 2020; DOI: 10.1126/scitranslmed.aaz3866

IgH recombination

Author: Masaki Miyazaki
Date: Sep 13, 2020，Views：871

Original protocol used in research article：

The transcription factor E2A activates multiple enhancers that drive Rag expression in developing T and B cells
Science Immunology, Sep 3, 2020; DOI: 10.1126/sciimmunol.abb1455

Synthesis and characterization of CpG-PP, CpG-PM, and CpG-P-ss-M

Authors: Huile Gao and Lin Qin
Date: Sep 10, 2020，Views：947

Original protocol used in research article：

A tumor-to-lymph procedure navigated versatile gel system for combinatorial therapy against tumor recurrence and metastasis
Science Advances, Sep 3, 2020; DOI: 10.1126/sciadv.abb3116

Quantitative RT-PCR analysis

Author: Katherine A Donovan
Date: Sep 10, 2020，Views：991

Original protocol used in research article：

Thalidomide promotes degradation of SALL4, a transcription factor implicated in Duane Radial Ray syndrome
eLife, Aug 1, 2018; DOI: 10.7554/eLife.38430

Detection and quantitation of NAD+- and NADH-capped RNA in vivo: hybridization with a radiolabeled oligodeoxyribonucleotide probe

Author: Jeremy G Bird
Date: Sep 10, 2020，Views：935

Original protocol used in research article：

Highly efficient 5' capping of mitochondrial RNA with NAD⁺ and NADH by yeast and human mitochondrial RNA polymerase
eLife, Dec 12, 2018; DOI: 10.7554/eLife.42179

Histology and molecular phenotyping

Author: Magda Spella
Date: Sep 9, 2020，Views：939

Original protocol used in research article：

Club cells form lung adenocarcinomas and maintain the alveoli of adult mice
eLife, May 29, 2019; DOI: 10.7554/eLife.45571

Sample preparation - Elemental mapping

Author: Gawain McColl
Date: Sep 9, 2020，Views：907

Original protocol used in research article：

Changes in ferrous iron and glutathione promote ferroptosis and frailty in aging Caenorhabditis elegans
eLife, Jul 21, 2020; DOI: 10.7554/eLife.56580

Preparation of brain samples for immunofluorescence, immunoblot, and nucleic acid analysis

Author: Bradlee L. Heckmann
Date: Sep 8, 2020，Views：845

Original protocol used in research article：

Noncanonical function of an autophagy protein prevents spontaneous Alzheimer’s disease
Science Advances, Aug 13, 2020; DOI: 10.1126/sciadv.abb9036

Thermostability shift assay and library screening

Author: Edmund R.S. Kunji
Date: Sep 7, 2020，Views：926

Original protocol used in research article：

Screening of candidate substrates and coupling ions of transporters by thermostability shift assays
eLife, Oct 15, 2018; DOI: 10.7554/eLife.38821

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