Bio-101 - Improve Research Reproducibility

Lysosome isolation and analysis

Authors: Yi Xu and Xiaolu Yang
Date: Sep 1, 2020，Views：2302

Original protocol used in research article：

Chaperone-mediated autophagy regulates the pluripotency of embryonic stem cells
Science, Jul 23, 2020; DOI: 10.1126/science.abb4467

Mt-mKeima-based mitophagy assay with FACS

Author: Koji Yamano
Date: Aug 31, 2020，Views：2083

Original protocol used in research article：

Endosomal Rab cycles regulate Parkin-mediated mitophagy
eLife, Jan 23, 2018; DOI: 10.7554/eLife.31326

Isolation of malaria hypnozoites and developing liver stage parasites by Flow Cytometric Cell sorting

Authors: Annemarie Voorberg - van der Wel, Sam O Hofman and Clemens H M Kocken
Date: Aug 31, 2020，Views：1184

Original protocol used in research article：

A comparative transcriptomic analysis of replicating and dormant liver stages of the relapsing malaria parasite Plasmodium cynomolgi
eLife, Dec 7, 2017; DOI: 10.7554/eLife.29605

MBD7 ChIP

Authors: Dongming Li, Xuemei Chen and Julie A Law
Date: Aug 31, 2020，Views：913

Original protocol used in research article：

The MBD7 complex promotes expression of methylated transgenes without significantly altering their methylation status
eLife, Apr 28, 2017; DOI: 10.7554/eLife.19893

Biodegradation of PLGA-MPs

Authors: Xueguang Lu and Ana Jaklenec
Date: Aug 28, 2020，Views：929

Original protocol used in research article：

Engineered PLGA microparticles for long-term pulsatile release of STING agonist for cancer immunotherapy
Science Translational Medicine, Aug 11, 2020; DOI: 10.1126/scitranslmed.aaz6606

RT-LAMP assay

Authors: Viet Loan Dao Thi, Michael Knop and Simon Anders
Date: Aug 28, 2020，Views：1193

Original protocol used in research article：

A colorimetric RT-LAMP assay and LAMP-sequencing for detecting SARS-CoV-2 RNA in clinical samples
Science Translational Medicine, Aug 12, 2020; DOI: 10.1126/scitranslmed.abc7075

S9.6 Slot blotting

Authors: Karlene A Cimprich and Madzia P Crossley
Date: Aug 27, 2020，Views：1619

Original protocol used in research article：

Co-transcriptional R-loops are the main cause of estrogen-induced DNA damage
eLife, Aug 23, 2016; DOI: 10.7554/eLife.17548

Measurement of transcriptional drift and drift-variance

Author: Michael Petrascheck
Date: Aug 27, 2020，Views：962

Original protocol used in research article：

Suppression of transcriptional drift extends C. elegans lifespan by postponing the onset of mortality
eLife, Dec 1, 2015; DOI: 10.7554/eLife.08833

Recombinant oligonucleosomes

Authors: Fangfang Jiao and Rui Guo
Date: Aug 27, 2020，Views：795

Original protocol used in research article：

RACK7 recognizes H3.3G34R mutation to suppress expression of MHC class II complex components and their delivery pathway in pediatric glioblastoma
Science Advances, Jul 16, 2020; DOI: 10.1126/sciadv.aba2113

PCR-RFLP of G1 and G2 mutations in the human Apolipoprotein-L1 (APOL1) gene

Author: Anneli Cooper
Date: Aug 25, 2020，Views：1370

Original protocol used in research article：

APOL1 renal risk variants have contrasting resistance and susceptibility associations with African trypanosomiasis
eLife, May 24, 2017; DOI: 10.7554/eLife.25461

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