Bio-101 - Improve Research Reproducibility

In vitro 2d matrigel migration and protrusion assay

Authors: Monte M Winslow, Julien Sage and Dian Yang
Date: Sep 6, 2020，Views：1022

Original protocol used in research article：

Axon-like protrusions promote small cell lung cancer migration and metastasis
eLife, Dec 13, 2019; DOI: 10.7554/eLife.50616

Anti-aggregation assays

Author: Tomohide Saio
Date: Sep 4, 2020，Views：967

Original protocol used in research article：

Oligomerization of a molecular chaperone modulates its activity
eLife, May 1, 2018; DOI: 10.7554/eLife.35731

WorMotel preparation

Author: Christopher Fang-Yen
Date: Sep 4, 2020，Views：1329

Original protocol used in research article：

Longitudinal imaging of Caenorhabditis elegans in a microfabricated device reveals variation in behavioral decline during aging
eLife, May 31, 2017; DOI: 10.7554/eLife.26652

Yeast strains and growth media

Authors: Abbas Jariani, Lieselotte Vermeersch and Kevin J Verstrepen
Date: Sep 4, 2020，Views：1108

Original protocol used in research article：

A new protocol for single-cell RNA-seq reveals stochastic gene expression during lag phase in budding yeast
eLife, May 18, 2020; DOI: 10.7554/eLife.55320

scRNA-seq using the 10x Genomics platform

Authors: Abbas Jariani, Lieselotte Vermeersch and Kevin J Verstrepen
Date: Sep 4, 2020，Views：1538

Original protocol used in research article：

A new protocol for single-cell RNA-seq reveals stochastic gene expression during lag phase in budding yeast
eLife, May 18, 2020; DOI: 10.7554/eLife.55320

Zebrafish genotyping

Authors: Kelly R Monk and Xianhua Piao
Date: Sep 3, 2020，Views：888

Original protocol used in research article：

Microglial transglutaminase-2 drives myelination and myelin repair via GPR56/ADGRG1 in oligodendrocyte precursor cells
eLife, May 29, 2018; DOI: 10.7554/eLife.33385

Neonatal transverse sinus injections for whole-brain gene expression

Authors: Ali S Hamodi and Michael Crair
Date: Sep 2, 2020，Views：3015

Original protocol used in research article：

Transverse sinus injections drive robust whole-brain expression of transgenes
eLife, May 18, 2020; DOI: 10.7554/eLife.53639

Pair-wise phase consistency for LFP-LFP phase synchrony

Author: Lu Zhang
Date: Sep 2, 2020，Views：1937

Original protocol used in research article：

Sub-second dynamics of theta-gamma coupling in hippocampal CA1
eLife, Jul 29, 2019; DOI: 10.7554/eLife.44320

Subcellular fractionation and immunoblotting

Author: Miriam Braunstein
Date: Sep 1, 2020，Views：1142

Original protocol used in research article：

Mycobacterium tuberculosis SatS is a chaperone for the SecA2 protein export pathway
eLife, Jan 3, 2019; DOI: 10.7554/eLife.40063

Culture filtrate protein preparation

Author: Miriam Braunstein
Date: Sep 1, 2020，Views：1131

Original protocol used in research article：

Mycobacterium tuberculosis SatS is a chaperone for the SecA2 protein export pathway
eLife, Jan 3, 2019; DOI: 10.7554/eLife.40063

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