Bio-101 - Improve Research Reproducibility

Immunohistochemistry (IHC)

Author: Daniel Hasche
Date: Oct 1, 2020，Views：1641

Original protocol used in research article：

Expression of different L1 isoforms of Mastomys natalensis papillomavirus as mechanism to circumvent adaptive immunity
eLife, Aug 4, 2020; DOI: 10.7554/eLife.57626

Ovine model of osteochondral defect repair

Author: Frances Henson
Date: Sep 30, 2020，Views：806

Original protocol used in research article：

Agrin induces long-term osteochondral regeneration by supporting repair morphogenesis
Science Translational Medicine, Sep 1, 2020; DOI: 10.1126/scitranslmed.aax9086

Cryo-EM sample preparation

Authors: Byung-Gil Lee and Jan Löwe
Date: Sep 30, 2020，Views：1134

Original protocol used in research article：

Transport of DNA within cohesin involves clamping on top of engaged heads by Scc2 and entrapment within the ring by Scc3
eLife, Sep 15, 2020; DOI: 10.7554/eLife.59560

Immunoprecipitation and GST pull-down

Author: Arnaud Besson
Date: Sep 29, 2020，Views：1045

Original protocol used in research article：

p27^Kip1 promotes invadopodia turnover and invasion through the regulation of the PAK1/Cortactin pathway
eLife, Mar 13, 2017; DOI: 10.7554/eLife.22207

Measurement of heat production (thermogenesis) in cells using ERthermAC dye

Authors: Chih-Hao Wang and Yu-Hua Tseng
Date: Sep 29, 2020，Views：1545

Original protocol used in research article：

CRISPR-engineered human brown-like adipocytes prevent diet-induced obesity and ameliorate metabolic syndrome in mice
Science Translational Medicine, Aug 25, 2020; DOI: 10.1126/scitranslmed.aaz8664

Heparin-induced filament assembly of tau

Author: Wenjuan Zhang
Date: Sep 28, 2020，Views：1600

Original protocol used in research article：

Heparin-induced tau filaments are polymorphic and differ from those in Alzheimer’s and Pick’s diseases
eLife, Feb 5, 2019; DOI: 10.7554/eLife.43584

Flow cytometry

Authors: Christian Perez-Shibayama, Cristina Gil-Cruz and Burkhard Ludewig
Date: Sep 24, 2020，Views：936

Original protocol used in research article：

Type I interferon signaling in fibroblastic reticular cells prevents exhaustive activation of antiviral CD8+ T cells
Science Immunology, Sep 11, 2020; DOI: 10.1126/sciimmunol.abb7066

Droplet-based scRNA-seq analysis

Authors: Christian Perez-Shibayama, Mechthild Luedge and Burkhard Ludewig
Date: Sep 24, 2020，Views：869

Original protocol used in research article：

Type I interferon signaling in fibroblastic reticular cells prevents exhaustive activation of antiviral CD8+ T cells
Science Immunology, Sep 11, 2020; DOI: 10.1126/sciimmunol.abb7066

RT-LAMP assay

Authors: Viet Loan Dao Thi, Michael Knop and Simon Anders
Date: Sep 24, 2020，Views：886

Original protocol used in research article：

A colorimetric RT-LAMP assay and LAMP-sequencing for detecting SARS-CoV-2 RNA in clinical samples
Science Translational Medicine, Aug 12, 2020; DOI: 10.1126/scitranslmed.abc7075

Untargeted metabolomics

Author: Carlos Guijas
Date: Sep 23, 2020，Views：922

Original protocol used in research article：

Metabolic adaptation to calorie restriction
Science Signaling, Sep 8, 2020; DOI: 10.1126/scisignal.abb2490

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