Bio-101 - Improve Research Reproducibility

Rubicon ThruPlex DNA-Seq library preparation protocol

Author: Laura A Lavery
Date: Aug 18, 2020，Views：1017

Original protocol used in research article：

Losing Dnmt3a dependent methylation in inhibitory neurons impairs neural function by a mechanism impacting Rett syndrome
eLife, Mar 11, 2020; DOI: 10.7554/eLife.52981

Western blotting

Author: Daniel T Hughes
Date: Aug 18, 2020，Views：905

Original protocol used in research article：

Targeting the kinase insert loop of PERK selectively modulates PERK signaling without systemic toxicity in mice
Science Signaling, Aug 10, 2020; DOI: 10.1126/scisignal.abb4749

Cell culture and treatment

Authors: Hyungsoo Kim and Ze’ev A. Ronai
Date: Aug 17, 2020，Views：946

Original protocol used in research article：

PRMT5 control of cGAS/STING and NLRC5 pathways defines melanoma response to antitumor immunity
Science Translational Medicine, Jul 7, 2020; DOI: 10.1126/scitranslmed.aaz5683

Preparation of SiUNPs, AUNPs, and pAUNPs

Author: Duo Mao
Date: Aug 17, 2020，Views：873

Original protocol used in research article：

AIEgen-coupled upconversion nanoparticles eradicate solid tumors through dual-mode ROS activation
Science Advances, Jun 25, 2020; DOI: 10.1126/sciadv.abb2712

In situ hybridization and antibody labeling

Author: Catherine M Drerup
Date: Aug 14, 2020，Views：938

Original protocol used in research article：

Regulation of mitochondria-dynactin interaction and mitochondrial retrograde transport in axons
eLife, Apr 17, 2017; DOI: 10.7554/eLife.22234

Construction and small-scale production of hIgG1Fc-VHH chimeric antibodies

Author: Dubravka Drabek
Date: Aug 12, 2020，Views：1038

Original protocol used in research article：

Multimeric single-domain antibody complexes protect against bunyavirus infections
eLife, Apr 21, 2020; DOI: 10.7554/eLife.52716

Synthesis of SCLMs

Author: Lingzhi Zhao
Date: Aug 12, 2020，Views：810

Original protocol used in research article：

MTH1 inhibitor amplifies the lethality of reactive oxygen species to tumor in photodynamic therapy
Science Advances, Mar 4, 2020; DOI: 10.1126/sciadv.aaz0575

Preparation and characterization of nanomedicine-shelled microbubbles

Authors: Yu Gao and Si yu Wang
Date: Aug 12, 2020，Views：851

Original protocol used in research article：

Accelerating thrombolysis using a precision and clot-penetrating drug delivery strategy by nanoparticle-shelled microbubbles
Science Advances, Jul 29, 2020; DOI: 10.1126/sciadv.aaz8204

Molecular dynamics simulations

Author: Gregory R Bowman
Date: Aug 12, 2020，Views：920

Original protocol used in research article：

Conformational distributions of isolated myosin motor domains encode their mechanochemical properties
eLife, May 29, 2020; DOI: 10.7554/eLife.55132

Quantitative Real-Time PCR

Author: Youngah Jo
Date: Aug 12, 2020，Views：957

Original protocol used in research article：

Schnyder corneal dystrophy-associated UBIAD1 inhibits ER-associated degradation of HMG CoA reductase in mice
eLife, Feb 20, 2019; DOI: 10.7554/eLife.44396

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