Bio-101 - Improve Research Reproducibility

Screening PETAL array with proteomic antigens

Author: Zhaohui Wang
Date: Jul 16, 2020，Views：957

Original protocol used in research article：

An array of 60000 antibodies for proteome-scale antibody generation and target discovery
Science Advances, Mar 10, 2020; DOI: 10.1126/sciadv.aax2271

In vitro release of Ad from NPs in serum

Author: Patrick Couvreur
Date: Jul 15, 2020，Views：948

Original protocol used in research article：

Squalene-based multidrug nanoparticles for improved mitigation of uncontrolled inflammation in rodents
Science Advances, Jun 4, 2020; DOI: 10.1126/sciadv.aaz5466

Proteinase K protection assay

Authors: Min Zhang and Lei Liu
Date: Jul 15, 2020，Views：3056

Original protocol used in research article：

Translocation of interleukin-1β into a vesicle intermediate in autophagy-mediated secretion
eLife, Nov 2, 2015; DOI: 10.7554/eLife.11205

Fluorescence quenching assay

Author: Mable Lam
Date: Jul 15, 2020，Views：995

Original protocol used in research article：

Misfolded proteins bind and activate death receptor 5 to trigger apoptosis during unresolved endoplasmic reticulum stress
eLife, Jan 6, 2020; DOI: 10.7554/eLife.52291

Peptide pulldown with Fc-tagged ECD proteins

Author: Mable Lam
Date: Jul 14, 2020，Views：999

Original protocol used in research article：

Misfolded proteins bind and activate death receptor 5 to trigger apoptosis during unresolved endoplasmic reticulum stress
eLife, Jan 6, 2020; DOI: 10.7554/eLife.52291

Kcr peptide enrichment

Authors: Zhongyi Cheng and Jing Liang
Date: Jul 14, 2020，Views：996

Original protocol used in research article：

Global crotonylome reveals CDYL-regulated RPA1 crotonylation in homologous recombination–mediated DNA repair
Science Advances, Mar 12, 2020; DOI: 10.1126/sciadv.aay4697

Lectin staining

Author: Katarina Valoskova
Date: Jul 14, 2020，Views：1403

Original protocol used in research article：

A conserved major facilitator superfamily member orchestrates a subset of O-glycosylation to aid macrophage tissue invasion
eLife, Mar 26, 2019; DOI: 10.7554/eLife.41801

Immunofluorescence staining – quantification of activated-caspase 3-positive cells in brain slices:

Authors: Oriane Blanquie, Heiko J Luhmann, Jenq-Wei Yang, Werner Kilb, Salim Sharopov and Anne Sinning
Date: Jul 14, 2020，Views：1337

Original protocol used in research article：

Electrical activity controls area-specific expression of neuronal apoptosis in the mouse developing cerebral cortex
eLife, Aug 21, 2017; DOI: 10.7554/eLife.27696

Lung tumor volume measurements from X-ray micro-computed tomography (μCT) scans

Authors: Caroline Contat and Etienne Meylan
Date: Jul 14, 2020，Views：1173

Original protocol used in research article：

Combined deletion of Glut1 and Glut3 impairs lung adenocarcinoma growth
eLife, Jun 23, 2020; DOI: 10.7554/eLife.53618

2-NBDG uptake

Authors: Kenneth Kin Lam Wong and Esther M Verheyen
Date: Jul 13, 2020，Views：1188

Original protocol used in research article：

A positive feedback loop between Myc and aerobic glycolysis sustains tumor growth in a Drosophila tumor model
eLife, Jul 1, 2019; DOI: 10.7554/eLife.46315

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