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A high-quality database for basic life science protocols
实验方法
研究领域
癌症生物学 (401)
发育生物学 (290)
分子生物学 (1016)
干细胞 (219)
免疫学 (564)
神经科学 (613)
生物化学 (1258)
生物物理学 (123)
微生物学 (1061)
系统生物学 (283)
细胞生物学 (1895)
植物科学 (983)
Bio-protocol 期刊
特刊
课题组实验方法合辑
Request a Protocol
提交
提交实验方法
作者指南
关于
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In vitro permeability assay
Authors:
Shima Ghoroghi
,
Jacky G Goetz
and
Vincent Hyenne
Date: May 6, 2021,Views:1227
Original protocol used in research article:
Ral GTPases promote breast cancer metastasis by controlling biogenesis and organ targeting of exosomes
eLife, Jan 6, 2021; DOI:
10.7554/eLife.61539
IP and MS
Author:
Xiaoming Zhang
Date: May 6, 2021,Views:931
Original protocol used in research article:
DEAD-box helicases modulate dicing body formation in Arabidopsis
Science Advances, Apr 28, 2021; DOI:
10.1126/sciadv.abc6266
Single-cell tissue preparation
Authors:
Kristen L Wells
,
Mark S Anderson
,
Lars M Steinmetz
and
Corey N Miller
Date: May 4, 2021,Views:880
Original protocol used in research article:
Combined transient ablation and single-cell RNA-sequencing reveals the development of medullary thymic epithelial cells
eLife, Nov 23, 2020; DOI:
10.7554/eLife.60188
Animal husbandry and handling
Authors:
Laura A Lavery
and
Huda Y Zoghbi
Date: May 4, 2021,Views:846
Original protocol used in research article:
Losing Dnmt3a dependent methylation in inhibitory neurons impairs neural function by a mechanism impacting Rett syndrome
eLife, Mar 11, 2020; DOI:
10.7554/eLife.52981
Integrative RNA-seq and methylation analysis
Authors:
Alexander Trostle
,
Laura A Lavery
,
Ying-wooi Wan
and
Huda Y Zoghbi
Date: May 4, 2021,Views:836
Original protocol used in research article:
Losing Dnmt3a dependent methylation in inhibitory neurons impairs neural function by a mechanism impacting Rett syndrome
eLife, Mar 11, 2020; DOI:
10.7554/eLife.52981
Animal experiments
Authors:
Hyungsoo Kim
and
Ze’ev A. Ronai
Date: May 3, 2021,Views:880
Original protocol used in research article:
PRMT5 control of cGAS/STING and NLRC5 pathways defines melanoma response to antitumor immunity
Science Translational Medicine, Jul 7, 2020; DOI:
10.1126/scitranslmed.aaz5683
Real-time place preference – RTPP
Authors:
Bárbara Coimbra
and
Ana João Rodrigues
Date: Apr 29, 2021,Views:838
Original protocol used in research article:
Impairments in laterodorsal tegmentum to VTA projections underlie glucocorticoid-triggered reward deficits
eLife, Aug 24, 2017; DOI:
10.7554/eLife.25843
Binding energy calculations by molecular mechanics–generalized Born surface area
Authors:
D. Collias
,
R. T. Leenay
,
R. A. Slotkowski
,
Z. Zuo
,
J. Liu
and
C. L. Beisel
Date: Apr 29, 2021,Views:932
Original protocol used in research article:
A positive growth-based PAM screen identifies noncanonical motifs recognized by the S. pyogenes Cas9
Science Advances, Jul 14, 2020; DOI:
10.1126/sciadv.abb4054
Dual RNA-seq sample preparation
Authors:
Gili Rosenberg
and
Roi Avraham
Date: Apr 29, 2021,Views:923
Original protocol used in research article:
Host succinate is an activation signal for Salmonella virulence during intracellular infection
Science, Jan 22, 2021; DOI:
10.1126/science.aba8026
Whisker and other hair trimming
Authors:
Kyle S Severson
and
Daniel H O'Connor
Date: Apr 28, 2021,Views:830
Original protocol used in research article:
Coding of whisker motion across the mouse face
eLife, Feb 28, 2019; DOI:
10.7554/eLife.41535
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